CXCR2/IL-8RB Antibody (866614) [Allophycocyanin]

Rat peripheral blood granulocytes were stained with Mouse Anti-Rat CXCR2/IL-8 RB APC-conjugated Monoclonal Antibody (Catalog # FAB8110A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

HEK293 human embryonic kidney cell line transfected with either (A) rat CXCR2/IL-8 RB or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Rat CXCR2/IL-8 RB APC-conjugated Monoclonal Antibody (Catalog # FAB8110A). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Rt
• Applications: Flow
• Clone: 866614
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

CXCR2/IL-8RB Antibody (866614) [Allophycocyanin] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with rat CXCR2/IL-8 RB
  Accession # P35407
• Specificity: Detects rat CXCR2/IL-8 RB in ELISA. Stains rat CXCR2/IL-8 RB transfected cells but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: Cxcr2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR2/IL-8RB Antibody (866614) [Allophycocyanin]

• CD182 antigen
• CD182
• CDw128b
• chemokine (C-X-C motif) receptor 2
• chemokine (CXC) receptor 2
• CMKAR2
• C-X-C chemokine receptor type 2
• CXCR2 gene for IL8 receptor type B
• CXCR2
• CXC-R2
• CXCR-2
• GRO/MGSA receptor
• High affinity interleukin-8 receptor B
• IL-8 RB
• IL-8 receptor type 2
• IL-8R B
• IL8R2
• IL8RA
• IL8RB
• interleukin 8 receptor B
• interleukin 8 receptor type 2
• interleukin-8 receptor type B

Background

CXCR2 is an approximately 40 kDa 7-transmembrane domain receptor for the ELR+ chemokines CXCL1, 2, 3, 5, 6, 7, 8 and MIF. CXCR2 is expressed on neutrophils, monocytes, eosinophils, basophils, mast cells, T cells, oligodendrocytes, airway smooth muscle cells, and vascular endothelial cells. It is additionally upregulated in several cancers. CXCR2 can associate into homodimers or heterodimers with CXCR1, CXCR4, CD74, or the delta Opioid Receptor. CXCR2 plays an important role in attracting immune cells to sites of inflammation followed by their adhesion and extravasation. It is also involved in angiogenesis, the development of inflammatory disorders, and cancer. Rat CXCR2 shares 71% and 86% amino acid sequence identity with human and mouse CXCR2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR2/IL-8RB Antibody (FAB8110A)(1)

Publication using FAB8110A

• S Li, X Zheng, H Li, J Zheng, X Chen, W Liu, Y Tai, Y Zhang, G Wang, Y Yang Mesenchymal Stem Cells Ameliorate Hepatic Ischemia/Reperfusion Injury via Inhibition of Neutrophil Recruitment J Immunol Res, 2018-12-03;2018(0):7283703. 2018-12-03 [PMID: 30622980] (Flow Cytometry, Rat)

Bioinformatics

• Gene Symbol: Cxcr2
• Uniprot:
  • P35407()