CXCR1/IL-8RA Antibody (1122A) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR1/IL-8 RA or (B) mouse CXCR2/IL-8 RB and eGFP was stained with Rabbit Anti-Mouse CXCR1/IL-8 RA Monoclonal Antibody (Catalog # MAB8628). Quadrant markers were set based on control antibody staining (Catalog # AB-105-C). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 1122A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CXCR1/IL-8RA Antibody (1122A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse CXCR1/IL‑8 RA Peptide
  Accession # Q810W6
• Specificity: Detects mouse CXCR1 transfectants but not CXCR2 transfectants by flow cytometry.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CXCR1
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCR1/IL-8RA Antibody (1122A) [Unconjugated]

• CD128
• CD181 antigen
• CD181
• CDw128aC-C
• chemokine (C-X-C motif) receptor 1
• CKR-1
• CMKAR1
• C-X-C chemokine receptor type 1
• CXCR1
• CXC-R1
• CXCR-1
• High affinity interleukin-8 receptor A
• IL-8 RA
• IL-8 receptor type 1
• IL-8R A
• IL8R1
• IL8RA
• IL8RAC-C-CKR-1
• IL8RBA
• interleukin 8 receptor, alpha
• interleukin-8 receptor type 1
• interleukin-8 receptor type A

Background

CXCR1, also known as IL-8 RA and CD181, is an approximately 60 kDa 7TM glycoprotein that functions as a receptor for the chemokine CXCL8/IL-8. It is expressed on neutrophils, monocytes, CD8 T cells, FoxP3+ CD4 Treg cells, mast cells, neuronal and glial cells, vascular endothelial cells, and melanoma. CXCR1 forms homodimers and heterodimers with CXCR2/IL-8 RB. It can be cleaved from neutrophils in the lungs of cystic fibrosis patients to release fragments that promote CXCL8 production from airway epithelial cells. CXCR1 mediates neutrophil activation and chemotaxis to sites of inflammation as well as angiogenesis and melanoma invasiveness. Mouse CXCR1 shares 65% and 89% amino acid sequence identity with human and rat CXCR1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR1/IL-8RA Antibody (MAB8628)(1)

Publication using MAB8628

• Marie Vandestienne, Yujiao Zhang, Icia Santos-Zas, Rida Al-Rifai, Jeremie Joffre, Andreas Giraud et al. TREM-1 orchestrates angiotensin II–induced monocyte trafficking and promotes experimental abdominal aortic aneurysm Journal of Clinical Investigation 2021-01-19 [PMID: 33258804] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: CXCR1
• Uniprot:
  • Q810W6()