Recombinant Rat CXCL5/ENA-78 Protein

SDS-Page: Rat CXCL5 Protein [NBP2-35266]

• Reactivity: Rt
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Rat CXCL5/ENA-78 Protein Summary

• Description: A single, non-glycosylated polypeptide chain containing 93 amino acids corresponding to CXCL5/ENA-78 Source: E. coli

  Uniprot ID: P97885

  Amino Acid Sequence: APFSAMVATE LRCVCLTLAP RINPKMIANL EVIPAGPHCP KVEVIAKLKN QKDNVCLDPQ APLIKKVIQK ILGSENKKTK RNALALVRSA STQ

• Details of Functionality: CXCL5 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10-100 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CXCL5
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of CXCL5/ENA-78 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 10.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 2 x PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Rat CXCL5/ENA-78 Protein

• chemokine (C-C motif) ligand 5
• C-X-C motif chemokine 5
• CXCL5
• CXCL5/ENA-78
• ENA78
• ENA-78
• ENA-78(1-78)
• Epithelial-derived neutrophil-activating protein 78
• LIX/CXCL5
• Neutrophil-activating peptide ENA-78
• neutrophil-activating protein 78
• SCYB5ENA78

Background

CXCL5 is a small cytokine belonging to the CXC chemokine family. It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. The expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. Recombinant rat CXCL5 contains 93 amino acids which is a single, non-glycosylated polypeptide chain. Rat CXCL5 shares 57% amino acid sequence identity with human CXCL5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CXCL5
• Uniprot:
  • P97885()
  • P97885()
  • P97885()