Recombinant Human CXCL3/GRO gamma/CINC-2/DCIP-1 Protein Summary
A biologically active protein to CXCL3/GRO gamma/CINC-2/DCIP-1.
Source: E. coli
Amino Acid Sequence: ASVVTELRCQ CLQTLQGIHL KNIQSVNVRS PGPHCAQTEV IATLKNGKKA CLNPASPMVQ KIIEKILNKG STN
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
|Details of Functionality
GRO gamma Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/ml
| Protein/Peptide Type
Biologically Active Protein
> 97 % pure by SDS-PAGE and HPLC
Less than 1 EU/ug of endotoxin as determined by LAL method
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at 4C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
> 97 % pure by SDS-PAGE and HPLC
Briefly centrifuge vial prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < or = -20 C.
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
Alternate Names for Recombinant Human CXCL3/GRO gamma/CINC-2/DCIP-1 Protein
- chemokine (C-X-C motif) ligand 3
- CINC-2 alpha
- CINC-2 alpha/CXCL3
- CINC-2 beta
- CINC-2 beta/CXCL3
- C-X-C motif chemokine 3
- GRO gamma
- GRO3 oncogene
- Growth-regulated protein gamma
- Macrophage inflammatory protein 2-beta
- melanoma growth stimulatory activity gamma
- MGSA gamma
CXCL3 is belonging to the CXC chemokine family, which is also known as CINC-2 in rat, DCIP-1 in murine, and GRO gamma in humans. The functional receptor for CXCL3 has been identified as CXCR2. Similar to other GRO proteins, CXCL3 is potent neutrophil attractants and activators. This chemokine were originally purified from the conditioned medium of rat granulation tissue. In rat, CINC-2 has two isoforms, known as CXCL3alpha/CINC-2alpha and CXCL3beta/CINC-2beta. CXCL3alpha differs from CXCL3beta as DKSS to PSL (98-101) at carboxy-terminal residues.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed
for 2 years from date of receipt.
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Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
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