CXCL3/GRO gamma/CINC-2/DCIP-1 Antibody (83504) [Unconjugated]

Recombinant Rat CXCL3/CINC‑2 alpha (Catalog # 516-CA) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL3/CINC‑2 alpha (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Rat CXCL3/CINC‑2 alpha / beta Monoclonal Antibody (Catalog # MAB516). The ND50 is typically0.5‑2.5 µg/mL.

• Reactivity: Rt
• Applications: WB, B/N, ELISA(Cap)
• Clone: 83504
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CXCL3/GRO gamma/CINC-2/DCIP-1 Antibody (83504) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant rat CXCL3/CINC-2 alpha / beta
  Arg33-Ser100
  Accession # Q10746
• Specificity: Detects rat CXCL3/CINC-2 alpha / beta in ELISAs and Western blots. In sandwich immunoassays, 27.9% cross‑reactivity with recombinant rat (rr) CINC-3 is observed and no cross-reactivity or interference with rrCINC‑1, recombinant mouse (rm) KC, rmMIP-2, or recombinant human GRO alpha is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: Cxcl3
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • ELISA Standard (Matched Pair)
  • Neutralization 0.5-2.5 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Rat CXCL3/CINC-2alpha /beta Biotinylated Antibody (Catalog number BAF516)
  Standard: Recombinant Rat CXCL3/GROgamma /CINC-2/DCIP-1 (Catalog number 517-CB)
  Standard: Recombinant Rat CXCL3/GROgamma /CINC-2/DCIP-1 (Catalog number 516-CA)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL3/GRO gamma/CINC-2/DCIP-1 Antibody (83504) [Unconjugated]

• chemokine (C-X-C motif) ligand 3
• CINC-2
• CINC-2b
• C-X-C motif chemokine 3
• CXCL3
• Dcip1
• Gm1960
• GRO gamma
• GRO3 oncogene
• GRO3
• GROG
• GRO-gamma
• GRO-gamma(1-73)
• Growth-regulated protein gamma
• Macrophage inflammatory protein 2-beta
• melanoma growth stimulatory activity gamma
• MGSA gamma
• MIP2B
• MIP-2b
• MIP2-beta
• SCYB3

Background

CINC-2 alpha and -2 beta are mRNA splice variant isoforms that differ by only 3 amino acids at their amino-termini. These proteins are CXC chemokines most closely related to human GROs and mouse MIP-2. They are produced by granulation tissue, activated macrophages and fibroblasts. CINC-2 biological activities are mediated by CXCR1 or CXCR2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL3/GRO gamma/CINC-2/DCIP-1 Antibody (MAB516)(1)

Publication using MAB516

• Miura TA, Wang J, Holmes KV, Mason RJ Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokines. Virology, 2007-09-05;369(2):288-98. 2007-09-05 [PMID: 17804032] (ELISA Development, Rat)

Bioinformatics

• Gene Symbol: Cxcl3
• Uniprot:
  • Q10746()