Recombinant Human CXCL2/GRO beta/MIP-2/CINC-3 Protein

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human CXCL2/GRO beta/MIP-2/CINC-3 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 73 amino acids corresponding to CXCL2/GRO beta/MIP-2/CINC-3 Source: E. coli

  Uniprot ID: P19875

  Amino Acid Sequence: APLATELRCQ CLQTLQGIHL KNIQSVKVKS PGPHCAQTEV IATLKNGQKA CLNPASPMVK KIIEKMLKNG KSN

• Details of Functionality: CXCL2 Protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CXCL2
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of CXCL2/GRO beta/MIP-2/CINC-3 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 7.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human CXCL2/GRO beta/MIP-2/CINC-3 Protein

• chemokine (C-X-C motif) ligand 2
• CINC-2a
• CINC3
• CINC-3
• CINC-3/CXCL2
• C-X-C Motif Chemokine 2
• CXCL2
• GRO beta
• GRO2 oncogene
• GRO2
• GROB
• Gro-beta
• Growth-regulated protein beta
• Macrophage Inflammatory Protein 2
• Macrophage inflammatory protein 2-alpha
• Macrophage Inflammatory Protein
• melanoma growth stimulatory activity beta
• MGSA beta
• MGSA-b
• MGSA-beta
• MIP2
• MIP-2
• MIP-2/CXCL2
• MIP2A
• MIP-2a
• MIP2-alpha
• SCYB2

Background

CXCL2 is a small cytokine belonging to the CXC chemokine family that is also known as Chemokine (C-X-C motif) ligand 2, and macrophage inflammatory protein 2-alpha (MIP2-alpha). CXCL2 is secreted by activated monocytes, neutrophils and macrophages and expressed at sites of inflammation. CXCL2 is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells and suppresses hematopoietic progenitor cell proliferation. Recombinant human CXCL2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CXCL2
• Uniprot:
  • P19875()
  • P19875()
  • P19875()