CXCL13/BLC/BCA-1 Antibody

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CXCL13/BLC/BCA‑1 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human CXCL13/BLC/BCA‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF801) at 10 µg/mL ...read more
Recombinant Human CXCL13/BLC/BCA‑1 (Catalog # 801-CX) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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CXCL13/BLC/BCA-1 Antibody Summary

Immunogen
E. coli-derived recombinant human CXCL13/BCA-1
Val23-Arg94
Accession # Q53X90
Specificity
Detects CXCL13/BLC/BCA‑1 in direct ELISAs and Western blots. 
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CXCL13
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 1-4 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF801 in the following applications:

Publications
Read Publications using
AF801 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL13/BLC/BCA-1 Antibody

  • ANGIE
  • ANGIE2
  • B cell-attracting chemokine 1
  • B lymphocyte chemoattractant
  • BCA1
  • BCA-1
  • BCA1B-cell chemoattractant
  • BCA-1CXC chemokine BLC
  • B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1)
  • BLC
  • BLCSmall-inducible cytokine B13
  • BLR1L
  • B-lymphocyte chemoattractant
  • chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant)
  • chemokine (C-X-C motif) ligand 13
  • C-X-C motif chemokine 13
  • CXCL13
  • SCYB13
  • SCYB13B-cell-attracting chemokine 1
  • small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)

Background

CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. BCA-1 cDNA encodes a protein of 109 amino acid residues with a leader sequence of 22 residues. Mature human BCA-1 shares 64% amino acid sequence similarity with the mouse protein and 23‑34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.
  1. Gunn, M.D. et al. (1998) Nature, 391:799.
  2. Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
  3. Forster, R. et al. (1996) Cell 87:1037.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF801
Species: Hu
Applications: WB, IHC, B/N

Publications for CXCL13/BLC/BCA-1 Antibody (AF801)(13)

We have publications tested in 4 confirmed species: Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque), Rabbit.

We have publications tested in 4 applications: ICC, IHC, IHC-Fr, IHC-P.


Filter By Application
ICC
(1)
IHC
(2)
IHC-Fr
(2)
IHC-P
(8)
All Applications
Filter By Species
Human
(10)
Mouse
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF801 Applications Species
HA Kim, YH Kim, YK Jeon, WI Yang, JE Kwon, JH Han Histopathology and expression of the chemokines CXCL10, CXCL13, and CXCR3 and the endogenous TLR-4 ligand S100A8/A9 in lymph nodes of patients with adult-onset Still&#039;s disease Sci Rep, 2019;9(1):7517. 2019 [PMID: 31101882] (IHC-P, Human) IHC-P Human
C Liu, X Huang, M Werner, R Broering, J Ge, Y Li, B Liao, J Sun, J Peng, M Lu, J Hou, X Zhang Elevated Expression of Chemokine CXCL13 in Chronic Hepatitis B Patients Links to Immune Control during Antiviral Therapy Front Immunol, 2017;8(0):323. 2017 [PMID: 28386259] (IHC, Human) IHC Human
Hif1a Deletion Reveals Pro-Neoplastic Function of B Cells in Pancreatic Neoplasia Cancer Discov, 2016;6(3):256-69. 2016 [PMID: 26715642] (IHC-P, Human) IHC-P Human
Clement M, Guedj K, Andreata F, Morvan M, Bey L, Khallou-Laschet J, Gaston A, Delbosc S, Alsac J, Bruneval P, Deschildre C, Le Borgne M, Castier Y, Kim H, Cantor H, Michel J, Caligiuri G, Nicoletti A Control of the T follicular helper-germinal center B-cell axis by CD8(+) regulatory T cells limits atherosclerosis and tertiary lymphoid organ development. Circulation, 2015;131(6):560-70. 2015 [PMID: 25552357] (IHC-P, Human) IHC-P Human
Di Caro G, Bergomas F, Grizzi F, Doni A, Bianchi P, Malesci A, Laghi L, Allavena P, Mantovani A, Marchesi F Occurrence of tertiary lymphoid tissue is associated with T-cell infiltration and predicts better prognosis in early-stage colorectal cancers. Clin Cancer Res, 2014;20(8):2147-58. 2014 [PMID: 24523438] (IHC-P, Human) IHC-P Human
Slight S, Rangel-Moreno J, Gopal R, Lin Y, Fallert Junecko B, Mehra S, Selman M, Becerril-Villanueva E, Baquera-Heredia J, Pavon L, Kaushal D, Reinhart T, Randall T, Khader S CXCR5(+) T helper cells mediate protective immunity against tuberculosis. J Clin Invest, 2013;123(2):712-26. 2013 [PMID: 23281399] (IHC-P, Human) IHC-P Human
Kanzaki S, Yamaguchi A, Yamaguchi K, Kojima Y, Suzuki K, Koumitsu N, Nagashima Y, Nagahama K, Ehara M, Hirayasu Y, Ryo A, Aoki I, Yamanaka S Thymic alterations in GM2 gangliosidoses model mice. PLoS ONE, 2010;5(8):. 2010 [PMID: 20856892] (IHC-Fr, Mouse) IHC-Fr Mouse
Mature antigen-experienced T helper cells synthesize and secrete the B cell chemoattractant CXCL13 in the inflammatory environment of the rheumatoid joint. Arthritis Rheum., 2008;58(0):3377-87. 2008 [PMID: 18975336] (ICC, Human) ICC Human
Ramesh G, Borda JT, Dufour J, Kaushal D, Ramamoorthy R, Lackner AA, Philipp MT Interaction of the Lyme disease spirochete Borrelia burgdorferi with brain parenchyma elicits inflammatory mediators from glial cells as well as glial and neuronal apoptosis. Am. J. Pathol., 2008;173(5):1415-27. 2008 [PMID: 18832582] (IHC-Fr, Primate - Macaca mulatta (Rhesus Macaque)) IHC-Fr Primate - Macaca mulatta (Rhesus Macaque)
Sansonno D, Tucci FA, Troiani L, Lauletta G, Montrone M, Conteduca V, Sansonno L, Dammacco F Increased serum levels of the chemokine CXCL13 and up-regulation of its gene expression are distinctive features of HCV-related cryoglobulinemia and correlate with active cutaneous vasculitis. Blood, 2008;112(5):1620-7. 2008 [PMID: 18550853] (IHC-P, Human) IHC-P Human
Show All 13 Publications.

Reviews for CXCL13/BLC/BCA-1 Antibody (AF801) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF801:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 CXCL13/BLC/BCA-1 AF801
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reviewed by:
Anonymous
Human 01/16/2020
View

Summary

Sample TestedDiffuse large B cell lymphoma
SpeciesHuman
LotBAS0317102

Comments

CommentspH 9 antigen retrieval
 
reviewed by:
Anonymous
IHC-P Human 01/09/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 23778140
SpeciesHuman

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Secondary Antibodies

 

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Biotin BAF801

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Recent Reviews

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5
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4
2
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0
2
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1
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Anonymous
01/16/2020
Application:
Species: Human

 
Anonymous
01/09/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol CXCL13
Uniprot