Recombinant Mouse CXCL11/I-TAC Protein

• Reactivity: Mu
• Applications: Bioactivity
• Concentration: LYOPH

Recombinant Mouse CXCL11/I-TAC Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 79 amino acids corresponding to CXCL11/I-TAC Source: E. coli

  Uniprot ID: Q9JHH5

  Amino Acid Sequence: FLMFKQGRCL CIGPGMKAVK MAEIEKASVI YPSNGCDKVE VIVTMKAHKR QRCLDPRSKQ ARLIMQAIEK KNFLRRQNM

• Details of Functionality: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine CXCR3 transfected 293 cells is in a concentration of 10-100 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CXCL11
• Purity: >98%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of CXCL11/I-TAC as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 9.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 10 mM Sodium Citrate, pH 4.0, with 600 mM NaCl.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >98%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse CXCL11/I-TAC Protein

• beta-R1
• b-R1
• chemokine (C-X-C motif) ligand 11
• CXCL11
• H174
• H174IP9
• Interferon gamma-inducible protein 9
• Interferon-inducible T-cell alpha chemoattractant
• IP-9member 11
• ITAC
• I-TAC
• I-TACMGC102770
• SCYB9B
• small inducible cytokine subfamily B (Cys-X-Cys), member 9B
• Small-inducible cytokine B11

Background

Small inducible cytokine B11 precursor; small inducible cytokine subfamily B (Cys-X-Cys), member 9B; small inducible cytokine subfamily B (Cys-X-Cys), member 11. This protein is a CXC member of the chemokine superfamily. It induces a chemotactic response in activated T-cells and is the dominant ligand for CXC receptor-3. The gene encoding this protein contains 4 exons and at least three polyadenylation signals which might reflect cell-specific regulation of expression. IFN-gamma is a potent inducer of transcription of this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CXCL11