CXCL10/IP-10/CRG-2 Antibody (33036) [Unconjugated]

Recombinant Human CXCL10/IP‑10 (Catalog # 266‑IP) chemo­attracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL10/IP‑10 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL10/IP‑10 Mono­clonal Antibody (Catalog # MAB266). The ND50 is typically 0.5-2.0 µg/mL.

• Reactivity: Hu
• Applications: B/N, CyTOF-ready, ELISA(Cap), Flow
• Clone: 33036
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CXCL10/IP-10/CRG-2 Antibody (33036) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CXCL10/IP-10/CRG-2
  Val22-Pro98
  Accession # P02778.2
• Specificity: Detects human CXCL10/IP-10/CRG-2 in ELISAs.
• Details of Functionality: ActivityAssaywCitation not found None None None
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CXCL10
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Human CXCL10/IP-10/CRG-2 Biotinylated Antibody (Catalog number BAF266)
  Standard: Recombinant Human CXCL10/IP-10/CRG-2 (Catalog number 266-IP)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL10/IP-10/CRG-2 Antibody (33036) [Unconjugated]

• C7
• chemokine (C-X-C motif) ligand 10
• CRG2
• CRG-2
• CXCL10
• gIP-10
• IFI10
• INP10
• IP-10
• mob-1
• SCYB10

Background

CXCL10 was originally identified as an IFN-gamma -inducible gene in monocytes, fibroblasts and endothelial cells. It has since been shown that CXCL10 mRNA is also induced by LPS, IL-1 beta, TNF-alpha, IL-12, and viruses. Additional cell types that have been shown to express CXCL10 include activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. CXCL10 is also expressed in psoriatic and lepromatous lesions of skin. The mouse homologue of human CXCL10, CRG-2, has been cloned and shown to share approximately 67% amino acid sequence identity with human CXCL10. Human CXCL10 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted protein. The amino acid sequence of CXCL10 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CXCL10 has been shown to be a chemoattractant for activated T-lymphocytes. CXCL10 has been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent antitumor effect. A chemokine receptor specific for CXCL10 and Mig has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
2. Wang, X. et al. (1996) J. Biol. Chem. 271:24286.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL10/IP-10/CRG-2 Antibody (MAB266)(30)

Publications using MAB266

• S Nishina, A Yamauchi, T Kawaguchi, K Kaku, M Goto, K Sasaki, Y Hara, Y Tomiyama, F Kuribayash, T Torimura, K Hino Dipeptidyl Peptidase 4 Inhibitors Reduce Hepatocellular Carcinoma by Activating Lymphocyte Chemotaxis in Mice Cell Mol Gastroenterol Hepatol, 2019;7(1):115-134. 2019 [PMID: 30510994] (Cell Selection, Mouse)
• Valentina Cecchinato Impairment of CCR6+ and CXCR3+ Th Cell Migration in HIV-1 Infection Is Rescued by Modulating Actin Polymerization J. Immunol, 2016;0(0):. 2016 [PMID: 27895171] (IHC-P, Primate - Macaca mulatta (Rhesus Macaque))
• Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus Sci Rep, 2016;6(0):29909. 2016 [PMID: 27453287] (Human)
• Schutte K, Fisher D, Burdick M, Mehrad B, Mathers A, Mann B, Nakamoto R, Hughes M Escherichia coli Pyruvate Dehydrogenase Complex Is an Important Component of CXCL10-Mediated Antimicrobial Activity. Infect Immun, 2015;84(1):320-8. 2015 [PMID: 26553462] (Immunoassay Development, Human)
• Pinto S, Martinez-Romero A, O'Connor J, Gil-Benso R, San-Miguel T, Terradez L, Monteagudo C, Callaghan R Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation. BMC Cancer, 2014;14(0):118. 2014 [PMID: 24559071] (Flow Cytometry, Human)
• Karta, Maya R, Wickert, Lisa E, Curran, Colleen, Gavala, Monica L, Denlinger, Loren C, Gern, James E, Bertics, Paul J Allergen challenge in vivo alters rhinovirus-induced chemokine secretion from human airway macrophages. J Allergy Clin Immunol, 2014;133(4):1227-30. 2014 [PMID: 24529411] (ELISA Development (Capture), Human)
• Zaheer R, Wiehler S, Hudy M, Traves S, Pelikan J, Leigh R, Proud D Human rhinovirus-induced ISG15 selectively modulates epithelial antiviral immunity. Mucosal Immunol, 2014;7(5):1127-38. 2014 [PMID: 24448099] (ELISA Development (Capture), Human)
• Srivastava R, Lee S, Andrade Filho P, Lord C, Jie H, Davidson H, Lopez-Albaitero A, Gibson S, Gooding W, Ferrone S, Ferris R Cetuximab-activated natural killer and dendritic cells collaborate to trigger tumor antigen-specific T-cell immunity in head and neck cancer patients. Clin Cancer Res, 2013;19(7):1858-72. 2013 [PMID: 23444227] (Functional Assay, Human)
• Nakajima N, Van Tin N, Sato Y, Thach H, Katano H, Diep P, Kumasaka T, Thuy N, Hasegawa H, San L, Kawachi S, Liem N, Suzuki K, Sata T Pathological study of archival lung tissues from five fatal cases of avian H5N1 influenza in Vietnam. Mod Pathol, 2012;26(3):357-69. 2012 [PMID: 23174938] (IHC-P, Human)
• Macrophages induce differentiation of plasma cells through CXCL10/IP-10. J. Exp. Med., 2012;209(10):1813-23. 2012 [PMID: 22987802] (Neutralization, Human)
• Zahn S, Rehkamper C, Kummerer BM, Ferring-Schmidt S, Bieber T, Tuting T, Wenzel J Evidence for a pathophysiological role of keratinocyte-derived type III interferon (IFN-lambda) in cutaneous lupus erythematosus. J. Invest. Dermatol., 2011;131(1):133-40. 2011 [PMID: 20720564] (IHC-P, Human)
• Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver. J. Immunol., 2010;184(6):2886-98. 2010 [PMID: 20164417] (Neutralization, Human)
• Yoon KC, Park CS, You IC, Choi HJ, Lee KH, Im SK, Park HY, Pflugfelder SC Expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome. Invest. Ophthalmol. Vis. Sci., 2010;51(2):643-50. 2010 [PMID: 19850844] (IHC-P, Human)
• Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadiere C, Mami-Chouaib F Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention. Cancer Res., 2009;69(15):6249-55. 2009 [PMID: 19638592] (ICC, Human)
• Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo. AIDS Res. Hum. Retroviruses, 2008;24(3):453-62. 2008 [PMID: 18327985] (Luminex Development, Human)
• Qui H, Ferrell EP, Nolan N, Phelps BH, Tabibiazar R, Whitney DH, Naelfski EA Fluorescence single-molecule counting assays for high-sensitivity detection of cytokines and chemokines. Clin. Chem., 2007;53(11):2010-2. 2007 [PMID: 18030696] (ELISA Development, Human)
• Pfleger C, Schloot N, ter Veld F Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. J. Immunol. Methods, 2007;329(1):214-8. 2007 [PMID: 17964594] (Luminex Development, Human)
• Cheadle EJ, Riyad K, Subar D, Rothwell DG, Ashton G, Batha H, Sherlock DJ, Hawkins RE, Gilham DE Eotaxin-2 and colorectal cancer: a potential target for immune therapy. Clin. Cancer Res., 2007;13(19):5719-28. 2007 [PMID: 17908961] (ELISA Development, Human)
• Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha. J. Immunol., 2007;179(7):4840-8. 2007 [PMID: 17878383] (ELISA Development, Human)
• Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients. J. Immunol., 2007;179(6):4272-82. 2007 [PMID: 17785868] (Immunodepletion, Human)
• Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1. AIDS, 2007;21(10):1263-72. 2007 [PMID: 17545702] (Luminex Development, Human)
• Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1. Blood, 2007;109(10):4272-9. 2007 [PMID: 17289812] (Luminex Development, Human)
• Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. Clin. Immunol., 2006;120(2):220-31. 2006 [PMID: 16782408] (Neutralization, Human)
• Edwards MR, Johnson MW, Johnston SL Combination therapy: Synergistic suppression of virus-induced chemokines in airway epithelial cells. Am. J. Respir. Cell Mol. Biol., 2006;34(5):616-24. 2006 [PMID: 16424382] (ELISA Development, Human)
• Wenzel J, Worenkamper E, Freutel S, Henze S, Haller O, Bieber T, Tuting T Enhanced type I interferon signalling promotes Th1-biased inflammation in cutaneous lupus erythematosus. J. Pathol., 2005;205(4):435-42. 2005 [PMID: 15685590] (ICC, Human)
• Sui Y, Li S, Pinson D, Adany I, Li Z, Villinger F, Narayan O, Buch S Simian human immunodeficiency virus-associated pneumonia correlates with increased expression of MCP-1, CXCL10, and viral RNA in the lungs of rhesus macaques. Am. J. Pathol., 2005;166(2):355-65. 2005 [PMID: 15681820] (Neutralization, Primate - Macaca mulatta (Rhesus Macaque))
• Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands. J. Immunol., 2004;173(11):6592-602. 2004 [PMID: 15557149] (IHC, Human)
• Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments. Blood, 2004;104(2):314-20. 2004 [PMID: 15044249] (ELISA Development, Human)
• Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells. J. Immunol., 2002;169(9):5244-51. 2002 [PMID: 12391243] (ELISA Development, Human)
• Lauw FN, Dekkers PE, te Velde AA, Speelman P, Levi M, Kurimoto M, Hack CE, Van Deventer SJ, van der Poll T Interleukin-12 induces sustained activation of multiple host inflammatory mediator systems in chimpanzees. J. Infect. Dis., 1999;179(3):646-52. 1999 [PMID: 9952371] (ELISA Development, Primate - Pan troglodytes (Chimpanzee))

Bioinformatics

• Gene Symbol: CXCL10
• Uniprot:
  • P02778.2()