CXCL1/GRO alpha/KC/CINC-1 Antibody (2176C) [Unconjugated]

Western blot shows lysates of NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with LPS. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Rat CXCL1/GROa/KC/CINC-1 Monoclonal Antibody (Catalog # MAB5151) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CXCL1/GROa/KC/CINC-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Recombinant Rat CXCL1/CINC-1 (Catalog # 515-CN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL1/CINC-1 (4 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Rat CXCL1/CINC-1 Monoclonal Antibody (Catalog # MAB5151). The ND50 is typically 0.075‑45 µg/mL.

• Reactivity: Rt
• Applications: WB, B/N
• Clone: 2176C
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CXCL1/GRO alpha/KC/CINC-1 Antibody (2176C) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant rat CXCL1/GRO alpha /KC/CINC‑1
  Ala25-Lys96
  Accession # P14095
• Specificity: Detects rat CXCL1/GRO alpha /KC/CINC‑1 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.075-0.45 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL1/GRO alpha/KC/CINC-1 Antibody (2176C) [Unconjugated]

• CINC1
• CINC-1
• CXCL1
• FSP
• GRO alpha
• GRO1
• GROa
• KC
• MGSA
• MGSA-a
• MGSA-alpha
• NAP-3
• SCYB1

Background

Cytokine-induced Neutrophil Chemoattractant 1 (CINC-1) was originally purified from media conditioned by IL-1 beta stimulated rat kidney epithelioid cells (NRK-52E). On the basis of its protein and DNA sequences, CINC-1 is a member of the alpha (CXC) subfamily of chemokines, designated CXCL1. Three additional rat CXC chemokines (CINC-2 alpha , CINC-2 beta , CINC-3/MIP-2), sharing approximately 63-67% amino acid sequence identity with CINC-1, have been identified. The protein sequence of rat CINC-1 is also 68%, 71%, and 69% identical to that of human GRO-alpha , GRO-beta , and GRO-gamma , respectively. Based on their sequence homology, it has been suggested that CINCs are the rat counterpart of human GROs. CINC-1 is also the counterpart of mouse KC. Rat CINC-1 cDNA encodes a 96 amino acid residue precursor protein from which the amino-terminal 24 amino acid residues are cleaved to generate the mature CINC-1. Similar to other alpha chemokines, rat CINCs are potent neutrophil attractants and activators and have been shown to play an important role in the infiltration of neutrophils into inflammatory sites in rats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P14095()