CXCL1/GRO alpha/KC/CINC-1 Antibody (124014) [Unconjugated]

Recombinant Mouse CXCL1/KC (Catalog # 453-KC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL1/KC (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse CXCL1/KC Monoclonal Antibody (Catalog # MAB4531). The ND50 is typically 1-5 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N
• Clone: 124014
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

CXCL1/GRO alpha/KC/CINC-1 Antibody (124014) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse CXCL1/GRO alpha /KC/CINC-1
  Arg20-Lys96
  Accession # P12850
• Specificity: Detects mouse CXCL1 in Western blots. Does not cross-react with recombinant human CXCL2, 3, 5, 6, 8, 9, 10, 11, 12/SDF-1 alpha , 12/SDF-1 beta , 13, recombinant mouse CXCL2, 6, 9, 10, 11, 12/SDF-1 alpha , 13, 15, recombinant rat (rr) CXCL2, rrCXCL3/CINC-2 alpha , rrCXCL3/CINC-2 beta , rrCXCL5, or recombinant porcine CXCL8.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Cxcl1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 1-5 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL1/GRO alpha/KC/CINC-1 Antibody (124014) [Unconjugated]

• CINC1
• CINC-1
• CXCL1
• FSP
• GRO alpha
• GRO1
• GROa
• KC
• MGSA
• MGSA-a
• MGSA-alpha
• NAP-3
• SCYB1

Background

CXCL1/GRO alpha, also known as KC in mouse and CINC-1 in rat, is a member of the CXC family of chemokines. Human CXCL1/GRO alpha is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 57% and 59% amino acid sequence identity with the human protein, respectively. Genome-wide analysis suggests that the protein encoded by the mouse Cxcl3 gene is homologous to human CXCL1/GRO alpha protein. Human CXCL1/GRO alpha can be cleaved into three isoforms CXCL1/GRO alpha (aa 4-73), CXCL1/GRO alpha (aa 5-73), and CXCL1/GRO alpha (aa 6-73) which show higher activity than the full length protein. CXCL1/GRO alpha is secreted by mast cells and macrophages whereupon it acts as a chemoattractant for neutrophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL1/GRO alpha/KC/CINC-1 Antibody (MAB4531)(8)

Publications using MAB4531

• SE Logue, EP McGrath, P Cleary, S Greene, K Mnich, A Almanza, E Chevet, RM Dwyer, A Oommen, P Legembre, F Godey, EC Madden, B Leuzzi, J Obacz, Q Zeng, JB Patterson, R Jäger, AM Gorman, A Samali Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy Nat Commun, 2018-08-15;9(1):3267. 8/15/2018 [PMID: 30111846]
• Hong Zhang, Patrick Neuhöfer, Liang Song, Björn Rabe, Marina Lesina, Magdalena U. Kurkowski et al. IL-6 trans-signaling promotes pancreatitis-associated lung injury and lethality Journal of Clinical Investigation 2013-03-01 [PMID: 23426178]
• Aeffner F, Davis I Respiratory syncytial virus reverses airway hyperresponsiveness to methacholine in ovalbumin-sensitized mice. PLoS ONE, 2012-10-02;7(10):e46660. 2012-10-02 [PMID: 23056391] (Neutralization, Mouse)
• Proebstl D, Voisin MB, Woodfin A, Whiteford J, D'Acquisto F, Jones GE, Rowe D, Nourshargh S Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo. J. Exp. Med., 2012-05-21;209(6):1219-34. 2012-05-21 [PMID: 22615129] (IHC, Mouse)
• Kim J, Natarajan S, Vaickus LJ, Bouchard JC, Beal D, Cruikshank WW, Remick DG Diesel exhaust particulates exacerbate asthma-like inflammation by increasing CXC chemokines. Am. J. Pathol., 2011-10-01;179(6):2730-9. 2011-10-01 [PMID: 21967814] (Neutralization, Mouse)
• Breslow JM, Meissler JJ, Hartzell RR Innate immune responses to systemic Acinetobacter baumannii infection in mice: neutrophils, but not interleukin-17, mediate host resistance. Infect. Immun., 2011-05-16;79(8):3317-27. 2011-05-16 [PMID: 21576323] (Neutralization, Mouse)
• Zhao C, Sardella A, Chun J, Poubelle PE, Fernandes MJ, Bourgoin SG TNF-alpha promotes LPA1- and LPA3-mediated recruitment of leukocytes in vivo through CXCR2 ligand chemokines. J. Lipid Res., 2011-04-26;52(7):1307-18. 2011-04-26 [PMID: 21521824] (Neutralization, Mouse)
• Traylor ZP, Yu EN, Davis IC Respiratory syncytial virus induces airway insensitivity to beta-agonists in BALB/c mice. Am. J. Physiol. Lung Cell Mol. Physiol., 2009-12-04;298(3):L437-45. 2009-12-04 [PMID: 19965982] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: Cxcl1
• Uniprot:
  • P12850()