CXCL1/GRO alpha/KC/CINC-1 Antibody (1174A) [Unconjugated]

Mouse splenocytes either (A) treated with 1 μg/mL LPS and Brefeldin A for 4 hours or (B) untreated were stained with Rabbit Anti-Mouse CXCL1/GRO alpha /KC/CINC‑1 Monoclonal Antibody (Catalog # MAB4532) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110) and Rat Anti-Mouse TNF‑ alpha Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC410F). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).

• Reactivity: Mu
• Applications: Flow
• Clone: 1174A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CXCL1/GRO alpha/KC/CINC-1 Antibody (1174A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant mouse CXCL1/GRO alpha /KC/CINC‑1
  Asn29-Lys96
  Accession # P12850
• Specificity: Detects mouse CXCL1/GRO alpha /KC/CINC‑1 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: Cxcl1
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL1/GRO alpha/KC/CINC-1 Antibody (1174A) [Unconjugated]

• CINC1
• CINC-1
• CXCL1
• FSP
• GRO alpha
• GRO1
• GROa
• KC
• MGSA
• MGSA-a
• MGSA-alpha
• NAP-3
• SCYB1

Background

KC, a member of the alpha (CXC) chemokine subfamily, was initially identified as an immediate early gene induced in mouse fibroblasts by platelet‑derived growth factor. KC cDNA encodes a 96 amino acid (aa) residue precursor protein with a predicted secretory signal peptide that is removed to yield the mature protein. The protein sequence of mouse KC shows approximately 63% identity to that of mouse MIP-2. KC is also approximately 60% identical to the human GROs. It has been suggested that mouse KC and MIP-2 are the orthologs of the human GROs and rat CINCs. In addition to mouse fibroblasts, KC is expressed in macrophages and endothelial cells. Mouse KC is a potent neutrophil attractant and activator. The functional receptor for KC has been identified as CXCR2. Based on the pattern of KC expression in a number of inflammatory disease models, KC appears to have an important role in inflammation. KC was found to be involved in monocyte arrest on atherosclerotic endothelium and may also play a pathophysiological role in Alzheimer’s disease. Many chemokines are substrates for selective proteolysis at the amino-terminus by various proteases including dipeptidyl peptidase IV or matrix metalloproteases, resulting in truncated chemokine isoforms with different (both enhanced or reduced) bioactivities. The naturally occurring 68 aa N-terminal truncated isoform of mouse KC is reported to be a more potent synergistic growth stimulant for CFU-GM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL1/GRO alpha/KC/CINC-1 Antibody (MAB4532)(2)

Publications using MAB4532

• Chung, KP;Cheng, CN;Chen, YJ;Hsu, CL;Huang, YL;Hsieh, MS;Kuo, HC;Lin, YT;Juan, YH;Nakahira, K;Chen, YF;Liu, WL;Ruan, SY;Chien, JY;Plataki, M;Cloonan, SM;Carmeliet, P;Choi, AMK;Kuo, CH;Yu, CJ; Alveolar epithelial cells mitigate neutrophilic inflammation in lung injury through regulating mitochondrial fatty acid oxidation Nature communications 2024-08-22 [PMID: 39174557] (Immunohistochemistry, Transgenic Mouse)
• Xiu-xiu Liu, Lin Yang, Ling-xiao Shao, Yang He, Gang Wu, Yu-huan Bao et al. Endothelial Cdk5 deficit leads to the development of spontaneous epilepsy through CXCL1/CXCR2-mediated reactive astrogliosis Journal of Experimental Medicine 2020-01-06 [PMID: 31699822]

Bioinformatics

• Gene Symbol: Cxcl1
• Uniprot:
  • P12850()