Rat CX3CL1/Fractalkine ELISA Kit (Colorimetric) Summary
Description |
Quality control test: Standard Curve. Cx3cl1 (Rat) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of rat Cx3cl1. |
Sensitivity |
5 pg/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
CV% < 12% |
Intra-Assay |
CV% < 10% |
Sample Volume |
100 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
CX3CL1 |
Applications/Dilutions
Dilutions |
|
Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
Storage |
Store at -20 to -80C. Avoid freeze-thaw cycles. |
Kit Components
Components
|
- Assay Diluent (Item E): 15 ml of 5x concentrated buffer. For Detection Antibody (Item F) andHRP-Streptavidin concentrate (Item G) diluent.
- Cell lysate buffer (Item J): 5 ml 2x cell lysate buffer.
- Detection Antibody Fractalkine (Item F): 2 vial of biotinylated anti-Rat Fractalkine (each vial is enough to assay half microplate).
- Fractalkine Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-Rat Fractalkine.
- HRP-Streptavidin concentrate (Item G): 8 µl of 12,000x concentrated HRP-conjugated streptavidin.
- Sample Diluent Buffer (Item D): 10 ml of 5x concentrated buffer. For Standard/Sample (cell lysate/tissue lysate) diluent.
- Standards (Item C): 2 vials, recombinant Rat Fractalkine.
- Stop Solution (Item I): 8 ml of 2 M sulfuric acid.
- TMB One-Step Substrate Reagent (Item H): 12 ml of 3,3’,5,5’-tetramethylbenzidine (TMB) in buffered solution.
- Wash Buffer Concentrate (20x) (Item B): 25 ml of 20x concentrated solution.
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Rat CX3CL1/Fractalkine ELISA Kit (Colorimetric)
Background
CX3CL1, also known as fractalkine, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, neurotactin, small-inducible cytokine D1, FKN, NTT, and SCYD1, is a member of the intercrine delta family. The soluble form of CX3CL1 is chemotactic for monocytes and T-cells, but not for neutrophils. CX3CL1's membrane bound form promotes adhesion of the monocytes and T-cells to endothelial cells. Additionally, CX3CL1 binds to CX3CR1 and regulates adhesion and migration of leukocyte processes at the endothelium.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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FAQs for CX3CL1/Fractalkine ELISA Kit (KA1827). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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