Mouse CX3CL1/Fractalkine ELISA Kit (Colorimetric)

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ELISA: Mouse CX3CL1/Fractalkine ELISA Kit (Colorimetric) [KA0663] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA0663
    • Availability
      Product Discontinued

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Mouse CX3CL1/Fractalkine ELISA Kit (Colorimetric) Summary

Description
Cx3cl1 (Mouse) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of mouse Cx3cl1.
Standard Curve Range
312 to 20000 pg/mL
Sensitivity
<10 pg/mL
Assay Type
Sandwich ELISA
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CX3CL1

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification
Application Notes
Useful for quantification.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Kit Components

Components
  1. ABC diluent buffer: 12ml.
  2. Antibody diluent buffer: 12ml.
  3. Avidin-Biotin-Peroxidase Complex (ABC): 130µl, dilution 1:100.
  4. Biotinylated anti- mouse Fractalkine antibody: 130µl, dilution 1:100
  5. Lyophilized recombinant mouse Fractalkine standard: 10ng/tube×2.
  6. One 96-well plate precoated with anti- mouse Fractalkine antibody.
  7. Sample diluent buffer: 30 ml
  8. TMB color developing agent: 10ml.
  9. TMB stop solution: 10ml.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Mouse CX3CL1/Fractalkine ELISA Kit (Colorimetric)

  • ABCD-3
  • C3Xkine
  • chemokine (C-X3-C motif) ligand 1
  • CX3C membrane-anchored chemokine
  • CX3CL1
  • CXC3
  • CXC3C
  • FKN
  • Fractalkine
  • Neurotactin
  • neurotactin)
  • NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine
  • NTTSmall-inducible cytokine D1
  • SCYD1C-X3-C motif chemokine 1
  • small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Background

CX3CL1, also known as fractalkine, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, neurotactin, small-inducible cytokine D1, FKN, NTT, and SCYD1, is a member of the intercrine delta family. The soluble form of CX3CL1 is chemotactic for monocytes and T-cells, but not for neutrophils. CX3CL1's membrane bound form promotes adhesion of the monocytes and T-cells to endothelial cells. Additionally, CX3CL1 binds to CX3CR1 and regulates adhesion and migration of leukocyte processes at the endothelium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for CX3CL1/Fractalkine ELISA Kit (KA0663). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol CX3CL1