CX3CL1/Fractalkine Antibody

Immunocytochemistry/Immunofluorescence: CX3CL1/Fractalkine Antibody [NBP1-49539] - Analysis of CX3CL (green) in HeLa cells using NBP1-49539. Nuclei (blue) are counterstained using Hoechst 33258.

Immunohistochemistry: CX3CL1/Fractalkine Antibody [NBP1-49539] - Analysis of CX3CL in mouse brain.

Immunohistochemistry: CX3CL1/Fractalkine Antibody [NBP1-49539] - Analysis of CX3CL in mouse lung

• Reactivity: Hu, Mu
• Applications: ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.15 mg/ml

CX3CL1/Fractalkine Antibody Summary

• Immunogen: A genomic peptide made to an internal region of the human CX3CL protein (within residues 20-150). [Swiss-Prot P78423]
• Localization: Cell membrane; Single-pass type I membrane protein. (Processed fractalkine: Secreted).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CX3CL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:250
• Application Notes: This CX3CL1 antibody is useful for IHC and ICC. Immunohistochemistry-Frozen was reported in scientific literature.

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1.15 mg/ml
• Purity: Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for CX3CL1/Fractalkine Antibody

• ABCD-3
• C3Xkine
• chemokine (C-X3-C motif) ligand 1
• CX3C membrane-anchored chemokine
• CX3CL1
• CXC3
• CXC3C
• FKN
• Fractalkine
• Neurotactin
• neurotactin)
• NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine
• NTTSmall-inducible cytokine D1
• SCYD1C-X3-C motif chemokine 1
• small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Background

As the only member of intercrine delta/ CX3C family, CX3CR1 (C-X3-C motif chemokine 1) is a type I transmembrane protein with CX3C chemokine domain present on an extended glycosylated stalk and it share this unique membrane anchorage with CXCR6 ligand, CXCL16. CX3CL1 is constitutively shed from the cell surface by ADAM10 (A Disintegrin and Metalloprotease 10) and CX3CL1 cleavage is enhanced upon activation of the closely related protease TNFalpha-converting enzyme, TACE (ADAM-17). CX3CL1 is expressed in a wide variety of cell types including neurons, intestinal, airway, skin epithelium cells, airway smooth muscle cells, and endothelium. It is induced by TNF and IL1 in pulmonary endothelial cells and umbilical vein endothelial cells. CX3CL1 signals through CX3CR1 (the only receptor of CX3CL1) which is expressed on monocytes, natural killer cells, T cells and smooth muscle cells. The major function of CX3CL1's soluble form is to act as chemotactic for T-cells and monocytes, but not for neutrophils, whereas, the membrane-bound form promotes adhesion of those leukocytes to endothelial cells where it may play a role in regulating leukocyte adhesion and migration processes at the endothelium. In addition to its role in chemotaxis and adhesion of leukocytes, CX3CL1 implicates in survival of multiple cell types during homeostasis and inflammation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CX3CL1/Fractalkine Antibody (NBP1-49539)(1)

Publication using NBP1-49539

• Schenkel JM, Fraser KA, Vezys V, Masopust D. Sensing and alarm function of resident memory CD8+ T cells Nat Immunol 2013 Mar 31 [PMID: 23542740] (IHC-Fr, ICC/IF, Mouse)

FAQs for CX3CL1/Fractalkine Antibody (NBP1-49539). (Showing 1 - 1 of 1 FAQ).

1. I’ve purchased your CX3CL1 Antibody (NMP-49539). Could you please let me know what the concentration of this antibody is?
   • The current lot, A1, of our CX3CL1 antibody with catalogue number NBP1-49539 is 0.57mg/ml.

Bioinformatics

• Gene Symbol: CX3CL1
• Entrez:
  • 6376(Human)
  • 20312(Mouse)
• Uniprot:
  • O35188()
  • P78423()