CX3CL1/Fractalkine Antibody (81513) [Unconjugated]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 81513
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CX3CL1/Fractalkine Antibody (81513) [Unconjugated] Summary

• Immunogen: NS0 and E. coli-derived recombinant human CX3CL1/Fractalkine Chemokine Domain
  Gln25-Arg339 (Ser199Asn)
  Accession # P78423
• Specificity: Detects human CX3CL1/Fractalkine Chemokine Domain in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CX3CL1

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CX3CL1/Fractalkine Antibody (81513) [Unconjugated]

• ABCD-3
• C3Xkine
• chemokine (C-X3-C motif) ligand 1
• CX3C membrane-anchored chemokine
• CX3CL1
• CXC3
• CXC3C
• FKN
• Fractalkine
• Neurotactin
• neurotactin)
• NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine
• NTTSmall-inducible cytokine D1
• SCYD1C-X3-C motif chemokine 1
• small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Background

CX3CL1 is a type I membrane protein in which an extracellular chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CX3CL1/Fractalkine Antibody (MAB3651)(8)

Publications using MAB3651

• W Liu, M Molnar, C Garnham, H Benav, H Rask-Ander Macrophages in the Human Cochlea: Saviors or Predators-A Study Using Super-Resolution Immunohistochemistry Front Immunol, 2018;9(0):223. 2018 [PMID: 29487598] (IHC, Human)
• Suzuki F, Kubota T Serum level of soluble CX3CL1/fractalkine is elevated in patients with polymyositis and dermatomyositis, which is correlated with disease activity. Arthritis Res. Ther., 2012;14(2):R48. 2012 [PMID: 22394569] (IHC-P, Human)
• Huang YW, Su P, Liu GY, Crow MR, Chaukos D, Yan H, Robinson LA Constitutive endocytosis of the chemokine CX3CL1 prevents its degradation by cell surface metalloproteases. J. Biol. Chem., 2009;284(43):29644-53. 2009 [PMID: 19723636] (ICC, Human)
• Hurst LA, Bunning RA, Couraud PO, Romero IA, Weksler BB, Sharrack B, Woodroofe MN Expression of ADAM-17, TIMP-3 and fractalkine in the human adult brain endothelial cell line, hCMEC/D3, following pro-inflammatory cytokine treatment. J. Neuroimmunol., 2009;210(1):108-12. 2009 [PMID: 19324423] (ICC, Human)
• Marchesi F, Piemonti L, Fedele G, Destro A, Roncalli M, Albarello L, Doglioni C, Anselmo A, Doni A, Bianchi P, Laghi L, Malesci A, Cervo L, Malosio M, Reni M, Zerbi A, Di Carlo V, Mantovani A, Allavena P The chemokine receptor CX3CR1 is involved in the neural tropism and malignant behavior of pancreatic ductal adenocarcinoma. Cancer Res., 2008;68(21):9060-9. 2008 [PMID: 18974152] (ICC, Human)
• Truman LA, Ford CA, Pasikowska M, Pound JD, Wilkinson SJ, Dumitriu IE, Melville L, Melrose LA, Ogden CA, Nibbs R, Graham G, Combadiere C, Gregory CD CX3CL1/fractalkine is released from apoptotic lymphocytes to stimulate macrophage chemotaxis. Blood, 2008;112(13):5026-36. 2008 [PMID: 18799722] (Western Blot, Human)
• Popovic M, Laumonnier Y, Burysek L, Syrovets T, Simmet T Thrombin-induced expression of endothelial CX3CL1 potentiates monocyte CCL2 production and transendothelial migration. J. Leukoc. Biol., 2008;84(1):215-23. 2008 [PMID: 18436581] (Western Blot, Human)
• Volger OL, Fledderus JO, Kisters N, Fontijn RD, Moerland PD, Kuiper J, van Berkel TJ, Bijnens AP, Daemen MJ, Pannekoek H, Horrevoets AJ Distinctive expression of chemokines and transforming growth factor-beta signaling in human arterial endothelium during atherosclerosis. Am. J. Pathol., 2007;171(1):326-37. 2007 [PMID: 17591977] (IHC, Human)

Review for CX3CL1/Fractalkine Antibody (MAB3651) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Gareth Healey IF Human 01/18/2016

Summary

• Application: Immunofluorescence
• Sample Tested: Human HEC1A cells
• Species: Human

Blocking

• Blocking Details: 3% BSA in PBS, 1 hour at room temperature

Primary Anitbody

• Dilution Ratio: Diluted 1 in 200 in 3% BSA (in PBS), incubated for 2 hours at room temp

Secondary Antibody

• Secondary Description: Goat anti-mouse IgG, AlexFluor 488
• Secondary Manufacturer Cat#: A11001
• Secondary Concentration: 1 in 1000 dilution in 3% BSA

Details

• Detection Notes: Confocal microscopy, Zeiss LSM 710, Objective: 63x/1.40 Oil DIC M27, neg conts = unstained, primary only, secondary only
• Fixation Details: Fixed in 4% PFA for 20 min at 4C
• Wash Description: PBS

Bioinformatics

• Gene Symbol: CX3CL1
• Uniprot:
  • P78423()