CX3CL1/Fractalkine Antibody (81506R) [Unconjugated]

NS0 cell line transfected with human CX3CL1/Fractalkine was stained with Mouse Anti-Human CX3CL1/Fractalkine Monoclonal Antibody (Catalog # MAB3652R, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. View our protocol for Staining Intracellular Proteins.

Recombinant Human CX3CL1/Fractalkine (Catalog # 365-FR) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CX3CL1/Fractalkine (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB3652). The ND50 is typically 1-5 µg/mL.

• Reactivity: Hu
• Applications: B/N, CyTOF-ready, Flow
• Clone: 81506R
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CX3CL1/Fractalkine Antibody (81506R) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: NS0-derived and E. coli-derived Recombinant human CX3CL1/Fracktalkine
  Gln25-Arg339
  Accession # P78423
• Specificity: Detects human CX3CL1/Fractalkine in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CX3CL1/Fractalkine Antibody (81506R) [Unconjugated]

• ABCD-3
• C3Xkine
• chemokine (C-X3-C motif) ligand 1
• CX3C membrane-anchored chemokine
• CX3CL1
• CXC3
• CXC3C
• FKN
• Fractalkine
• Neurotactin
• neurotactin)
• NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine
• NTTSmall-inducible cytokine D1
• SCYD1C-X3-C motif chemokine 1
• small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Background

CX3CL1, also known as Fractalkine, is a type I membrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CX3CL1/Fractalkine Antibody (MAB3652R)(1)

Publication using MAB3652R

• Lisha Luo, Shuanglinzi Deng, Wei Tang, Xinyue Hu, Feifei Yin, Huan Ge et al. Monocytes subtypes from pleural effusion reveal biomarker candidates for the diagnosis of tuberculosis and malignancy Journal of Clinical Laboratory Analysis 2022-08-01 [PMID: 35819097]

Bioinformatics

• Uniprot:
  • P78423()