CX3CL1/Fractalkine Antibody (81506) [Unconjugated]

Recombinant Human CX3CL1/Fractalkine (Catalog # 365-FR) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CX3CL1/Fractalkine (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB3652). The ND50 is typically 1-5 µg/mL.

• Reactivity: Hu
• Applications: B/N, CyTOF-ready, ELISA(Cap), Flow
• Clone: 81506
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CX3CL1/Fractalkine Antibody (81506) [Unconjugated] Summary

• Immunogen: NS0-derived and E. coli-derived Recombinant Human CX3CL1/Fracktalkine
  Gln25-Arg339
  Accession # P78423
• Specificity: Detects human CX3CL1/Fractalkine in ELISAs. In sandwich immunoassays, less than 10% cross-reactivity with recombinant mouse CXCL6 and less than 0.2% cross-reactivity with recombinant human (rh) CXCL6, rhCXCL9, and recombinant rat CX3CL1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CX3CL1
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 1-5 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Detection: Human CX3CL1/Fractalkine Chemokine Domain Biotinylated Antibody (Catalog number BAM365)
  Standard: Recombinant Human CX3CL1/Fractalkine Full Length (Catalog number 365-FR)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CX3CL1/Fractalkine Antibody (81506) [Unconjugated]

• ABCD-3
• C3Xkine
• chemokine (C-X3-C motif) ligand 1
• CX3C membrane-anchored chemokine
• CX3CL1
• CXC3
• CXC3C
• FKN
• Fractalkine
• Neurotactin
• neurotactin)
• NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine
• NTTSmall-inducible cytokine D1
• SCYD1C-X3-C motif chemokine 1
• small inducible cytokine subfamily D (Cys-X3-Cys), member-1

Background

CX3CL1, also known as Fractalkine, is a type I membrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CX3CL1/Fractalkine Antibody (MAB3652)(9)

Publications using MAB3652

• M Brown, LA Johnson, DA Leone, P Majek, K Vaahtomeri, D Senfter, N Bukosza, H Schachner, G Asfour, B Langer, R Hauschild, K Parapatics, YK Hong, KL Bennett, R Kain, M Detmar, M Sixt, DG Jackson, D Kerjaschki Lymphatic exosomes promote dendritic cell migration along guidance cues J. Cell Biol., 2018;0(0):. 2018 [PMID: 29650776] (Neutralization, Human)
• A Babendreye, L Molls, D Dreymuelle, S Uhlig, A Ludwig Shear Stress Counteracts Endothelial CX3CL1 Induction and Monocytic Cell Adhesion Mediators Inflamm., 2017;2017(0):1515389. 2017 [PMID: 28522896] (ELISA Development (Capture), Human)
• N Quillinan, KE Clark, B Youl, J Vernes, D McIntosh, S Haq, CP Denton Multiplex serum protein analysis reveals potential mechanisms and markers of response to hyperimmune caprine serum in systemic sclerosis Arthritis Res. Ther, 2017;19(1):45. 2017 [PMID: 28270187] (ELISA Development (Capture), Human)
• Kassianos A, Wang X, Sampangi S, Afrin S, Wilkinson R, Healy H Fractalkine-CX3CR1-dependent recruitment and retention of human CD1c+ myeloid dendritic cells by in vitro-activated proximal tubular epithelial cells. Kidney Int, 2015;87(6):1153-63. 2015 [PMID: 25587706] (Neutralization, Human)
• Denoyer A, Godefroy D, Celerier I, Frugier J, Riancho L, Baudouin F, Rostene W, Baudouin C CX3CL1 expression in the conjunctiva is involved in immune cell trafficking during toxic ocular surface inflammation. Mucosal Immunol, 2012;5(0):702. 2012 [PMID: 22692452] (Flow Cytometry, Human)
• Nevo I, Sagi-Assif O, Meshel T, Ben-Baruch A, Johrer K, Greil R, Trejo LE, Kharenko O, Feinmesser M, Yron I, Witz IP The involvement of the fractalkine receptor in the transmigration of neuroblastoma cells through bone-marrow endothelial cells. Cancer Lett., 2009;273(1):127-39. 2009 [PMID: 18778890] (Flow Cytometry, Human)
• Popovic M, Laumonnier Y, Burysek L, Syrovets T, Simmet T Thrombin-induced expression of endothelial CX3CL1 potentiates monocyte CCL2 production and transendothelial migration. J. Leukoc. Biol., 2008;84(1):215-23. 2008 [PMID: 18436581] (Western Blot, Human)
• Hundhausen C, Schulte A, Schulz B, Andrzejewski MG, Schwarz N, von Hundelshausen P, Winter U, Paliga K, Reiss K, Saftig P, Weber C, Ludwig A Regulated shedding of transmembrane chemokines by the disintegrin and metalloproteinase 10 facilitates detachment of adherent leukocytes. J. Immunol., 2007;178(12):8064-72. 2007 [PMID: 17548644] (ELISA Development, Human)
• Lavergne E, Combadiere B, Bonduelle O, Iga M, Gao JL, Maho M, Boissonnas A, Murphy PM, Debre P, Combadiere C Fractalkine mediates natural killer-dependent antitumor responses in vivo. Cancer Res., 2003;63(21):7468-74. 2003 [PMID: 14612547] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: CX3CL1
• Uniprot:
  • P78423()