CTCF Antibody

Images

 
Western Blot: CTCF Antibody [NB100-56494] - Western blot in HeLa cells after siControl and siCTCF. Image from verified customer review.
Western Blot: CTCF Antibody [NB100-56494] - analysis of CTCF in nuclear fraction HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CTCF Antibody Summary

Immunogen
A portion of amino acids 650-700 of human CTCF was used as the immunogen.
Specificity
The amino acid sequence used as immunogen is 100% homologous in human (isoforms CRA_a, _b, _c, _d), rhesus monkey, dog, mouse and rat.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CTCF
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
Positive Control
HeLa Lysate (NB800-PC9)
Reviewed Applications
Read 1 Review rated 4
using
NB100-56494 in the following application:

Reactivity Notes

Cross reacts with Dog, Human, Mouse, Rat and Rhesus Monkey.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for CTCF Antibody

  • 11 zinc finger transcriptional repressor
  • 11-zinc finger protein
  • 11-Zinc Finger Protein2
  • CCCTC-binding factor (zinc finger protein)
  • CCCTC-binding Factor
  • CTCF
  • CTCFL paralog
  • transcriptional repressor CTCF

Background

Transcriptional factor CTCF is a ubiquitously expressed 11-zinc finger domain containing, DNA-binding nuclear phosphoprotein that is involved in multiple aspects of normal gene regulation including transcriptional repression and activation, gene silencing, chromatin insulation and regulation of imprinted sites. CTCF uses different combinations of ZF domains and interacts with the CCCTC motif on the DNA. It can bind to HAT- and HDAC-containing complexes thereby influencing transcriptional activation or repression respectively. It is a multifunctional protein involved in the transactivation of APPB gene, silencing of c-myc gene, and insulation of human beta-globin and DMI myotonic dystrophy locus and imprinting control of IGF2 and H19 regions. CTCF has also been shown to regulate the transcription of Pax6 and IRAK2 promoter thereby opening a therapeutic avenue. Mutations in this gene have been associated with invasive breast cancers, prostate cancers and Wilms' tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CTCF Antibody (NB100-56494) (0)

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Review for CTCF Antibody (NB100-56494) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56494:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot CTCF NB100-56494
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reviewed by:
Ranveer Singh
WB Human 06/11/2014
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Summary

ApplicationWestern Blot
Sample TestedHeLa cells whole cell lysate
SpeciesHuman
CommentsMolecular weight abnormality for CTCF is well known. This antibody also gives multiple bands but the most prominent one is 82KDa, predicted molecular weight of CTCF

Blocking

Blocking Details5% BSA in TBST for 1 h

Primary Anitbody

Dilution Ratio1:1000, Overnight at 4 degree in TBST on a rocker

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG HRP (Biorad)
Secondary Manufacturer Cat#170-5046
Secondary Concentration1:20,000 in 5% BSA

Details

Detection NotesECL reagent was used. Exposure for 1 minute

Comments

CommentsMolecular weight abnormality for CTCF is well known. This antibody also gives multiple bands but the most prominent one is 82KDa, predicted molecular weight of CTCF

Product General Protocols

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Video Protocols

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FAQs for CTCF Antibody (NB100-56494) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for CTCF Antibody (NB100-56494)

Discover related pathways, diseases and genes to CTCF Antibody (NB100-56494). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for CTCF Antibody (NB100-56494)

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Pathways for CTCF Antibody (NB100-56494)

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PTMs for CTCF Antibody (NB100-56494)

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Research Areas for CTCF Antibody (NB100-56494)

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Blogs on CTCF

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Ranveer Singh
06/11/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol CTCF
Entrez
Uniprot