CTCF Antibody

Product Discontinued

CTCF Antibody Summary

• Immunogen: A portion of amino acids 650-700 of human CTCF was used as the immunogen.
• Specificity: The amino acid sequence used as immunogen is 100% homologous in human (isoforms CRA_a, _b, _c, _d), rhesus monkey, dog, mouse and rat.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CTCF
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-3 ug/ml
• Control:

  HeLa Nuclear Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for CTCF Antibody

• 11 zinc finger transcriptional repressor
• 11-zinc finger protein
• 11-Zinc Finger Protein2
• CCCTC-binding factor (zinc finger protein)
• CCCTC-binding Factor
• CTCF
• CTCFL paralog
• transcriptional repressor CTCF

Background

Transcriptional factor CTCF is a ubiquitously expressed 11-zinc finger domain containing, DNA-binding nuclear phosphoprotein that is involved in multiple aspects of normal gene regulation including transcriptional repression and activation, gene silencing, chromatin insulation and regulation of imprinted sites. CTCF uses different combinations of ZF domains and interacts with the CCCTC motif on the DNA. It can bind to HAT- and HDAC-containing complexes thereby influencing transcriptional activation or repression respectively. It is a multifunctional protein involved in the transactivation of APPB gene, silencing of c-myc gene, and insulation of human beta-globin and DMI myotonic dystrophy locus and imprinting control of IGF2 and H19 regions. CTCF has also been shown to regulate the transcription of Pax6 and IRAK2 promoter thereby opening a therapeutic avenue. Mutations in this gene have been associated with invasive breast cancers, prostate cancers and Wilms' tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for CTCF Antibody (NB100-56494) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Ranveer Singh WB Human 06/11/2014

Summary

• Application: Western Blot
• Sample Tested: HeLa cells whole cell lysate
• Species: Human

Bioinformatics

• Gene Symbol: CTCF
• Entrez:
  • 10664(Human)
  • 13018(Mouse)
  • 83726(Rat)
• Uniprot:
  • P49711(Human)