CSRP2 Antibody

Images

 
Western Blot: CSRP2 Antibody [NB100-77341] - Analysis of Human (A), Mouse (B) and Rat (C) Kidney lysates (35ug protein in RIPA buffer).
Immunohistochemistry-Paraffin: CSRP2 Antibody [NB100-77341] - Analysis of Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: CSRP2 Antibody [NB100-77341] - HEK293 overexpressing CRP2 and probed with (mock transfection in first lane).

Product Details

Summary
Reactivity Hu, Mu, Rt, EqSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CSRP2 Antibody Summary

Immunogen
Peptide with sequence C-ERLGIKPESVQP corresponding to internal region according to NP_001312.1.
Epitope
C-ERLGIKPESVQP
Predicted Species
Equine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CSRP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:64000
Application Notes
WB: Approx. 26 kDa band observed in human heart and kidney lysates and in mouse and rat kidney lysates is reported in scientific literature (PMID: 10681529). In transfected HEK293 transiently expressing CRP2 a band of approx. 26 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human Lung shows staining of the endothelial cells of a blood vessel.
Control
CSRP2 Overexpression Lysate
Publications
Read Publications using NB100-77341.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CSRP2 Antibody

  • CRP2
  • CRP2LIM domain only protein 5
  • CSRP2
  • cysteine and glycine-rich protein 2
  • Cysteine-rich protein 2
  • LMO5LMO-5
  • SmLIM
  • SmLIMLIM domain only 5, smooth muscle
  • Smooth muscle cell LIM protein

Background

CSRP2 is a member of the CSRP family of genes, encoding a group of LIM domain proteins, which may be involved in regulatory processes important for development and cellular differentiation. CRP2 contains two copies of the cysteine-rich amino acid sequence motif (LIM) with putative zinc-binding activity, and may be involved in regulating ordered cell growth. Other genes in the family include CSRP1 and CSRP3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CSRP2 Antibody (NB100-77341)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol CSRP2
Entrez
Uniprot