CSRP2 Antibody

Western Blot: CSRP2 Antibody [NB100-77341] - Analysis of Human (A), Mouse (B) and Rat (C) Kidney lysates (35ug protein in RIPA buffer).

Immunohistochemistry-Paraffin: CSRP2 Antibody [NB100-77341] - Analysis of Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: CSRP2 Antibody [NB100-77341] - HEK293 overexpressing CRP2 and probed with (mock transfection in first lane).

• Reactivity: Hu, Mu, Rt, Eq
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CSRP2 Antibody Summary

• Description: Novus Biologicals Goat CSRP2 Antibody (NB100-77341) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-CSRP2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-ERLGIKPESVQP corresponding to internal region according to NP_001312.1.
• Epitope: C-ERLGIKPESVQP
• Predicted Species: Equine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CSRP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 26 kDa band observed in human heart and kidney lysates and in mouse and rat kidney lysates is reported in scientific literature (PMID: 10681529). In transfected HEK293 transiently expressing CRP2 a band of approx. 26 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human Lung shows staining of the endothelial cells of a blood vessel.
• Control:

  CSRP2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CSRP2 Antibody

• CRP2
• CRP2LIM domain only protein 5
• CSRP2
• cysteine and glycine-rich protein 2
• Cysteine-rich protein 2
• LMO5LMO-5
• SmLIM
• SmLIMLIM domain only 5, smooth muscle
• Smooth muscle cell LIM protein

Background

CSRP2 is a member of the CSRP family of genes, encoding a group of LIM domain proteins, which may be involved in regulatory processes important for development and cellular differentiation. CRP2 contains two copies of the cysteine-rich amino acid sequence motif (LIM) with putative zinc-binding activity, and may be involved in regulating ordered cell growth. Other genes in the family include CSRP1 and CSRP3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CSRP2 Antibody (NB100-77341)(2)

Publications using NB100-77341

• Stearns ME, Wang M, Hu Y, Garcia FU. Interleukin-10 activation of the interleukin-10E1 pathway and tissue inhibitor of metalloproteinase-1 expression is enhanced by proteasome inhibitors in primary prostate tumor lines. Mol Cancer Res 2003-07-01 [PMID: 12861049]
• Huber A, Neuhuber WL, Klugbauer N et al. Cysteine-rich protein 2, a novel substrate for cGMP kinase I in enteric neurons and intestinal smooth muscle. J Biol Chem 2000-02-01 [PMID: 10681529]

Bioinformatics

• Gene Symbol: CSRP2
• Entrez:
  • 1466(Human)
  • 13008(Mouse)
• Uniprot:
  • NP_001312.1(Human)