![Immunoprecipitation: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - CSNK1A1/CSNK1A1L was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-33146 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33146 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-33146 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-33146 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 11 seconds; ECL](http://images.novusbio.com/fullsize/nbp3-33146_rabbit-csnk1a1-csnk1a1l-mab-psh04-58-26820241582616.jpg "Immunoprecipitation: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - CSNK1A1/CSNK1A1L was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-33146 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33146 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: NBP3-33146 IP in HeLa cell lysate Lane 3: Rabbit IgG instead of NBP3-33146 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 11 seconds; ECL")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | PSH04-58 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Description | Novus Biologicals Rabbit CSNK1A1/CSNK1A1L Antibody (PSH04-58) (NBP3-33146) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein within human CSNK1A1 aa 1-337 / 337. (Uniprot: P48729) |
| Localization | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Chromosome, centromere, kinetochore, Nucleus speckle, cilium basal body, spindle. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CSNK1A1 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 39 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH7.4), 0.1% BSA and 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![Western Blot beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Western_Blot_5100.jpg)
![Chromatin Immunoprecipitation (ChIP) beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Chromatin_Immunoprecipitation_11251.jpg)
![Simple Western beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/betaCatenin_AF1329_Simple_Western_17134.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CSNK1A1 |
![Western Blot: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - Western blot analysis of CSNK1A1/CSNK1A1L on different lysates with Rabbit anti-CSNK1A1/CSNK1A1L antibody (NBP3-33146) at 1/2,000 dilution. Lane 1: HCT 116 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: RAW264.7 cell lysate (20 ug/Lane) Lane 8: PC-12 cell lysate (20 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Lane 10: Rat brain tissue lysate (40 ug/Lane) Lane 11: Mouse kidney tissue lysate (40 ug/Lane) Lane 12: Rat kidney tissue lysate (40 ug/Lane) Lane 13: Mouse spleen tissue lysate (40 ug/Lane) Lane 14: Rat spleen tissue lysate (40 ug/Lane) Predicted band size: 39 kDa Observed band size: 34 kDa Exposure time: 15 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33146) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33146_rabbit-csnk1a1-csnk1a1l-mab-psh04-58-28820241430401.jpg "Western Blot: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - Western blot analysis of CSNK1A1/CSNK1A1L on different lysates with Rabbit anti-CSNK1A1/CSNK1A1L antibody (NBP3-33146) at 1/2,000 dilution. Lane 1: HCT 116 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HEK-293 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: Jurkat cell lysate (20 ug/Lane) Lane 6: NIH/3T3 cell lysate (20 ug/Lane) Lane 7: RAW264.7 cell lysate (20 ug/Lane) Lane 8: PC-12 cell lysate (20 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Lane 10: Rat brain tissue lysate (40 ug/Lane) Lane 11: Mouse kidney tissue lysate (40 ug/Lane) Lane 12: Rat kidney tissue lysate (40 ug/Lane) Lane 13: Mouse spleen tissue lysate (40 ug/Lane) Lane 14: Rat spleen tissue lysate (40 ug/Lane) Predicted band size: 39 kDa Observed band size: 34 kDa Exposure time: 15 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33146) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-CSNK1A1/CSNK1A1L antibody (NBP3-33146) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33146) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33146_rabbit-csnk1a1-csnk1a1l-mab-psh04-58-29820241435251.jpg "Immunohistochemistry: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-CSNK1A1/CSNK1A1L antibody (NBP3-33146) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33146) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - Immunocytochemistry analysis of HCT 116 cells labeling CSNK1A1/CSNK1A1L with Rabbit anti-CSNK1A1/CSNK1A1L antibody (NBP3-33146) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CSNK1A1/CSNK1A1L antibody (NBP3-33146) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33146_rabbit-csnk1a1-csnk1a1l-mab-psh04-58-29202410234299.jpg "Immunocytochemistry/ Immunofluorescence: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - Immunocytochemistry analysis of HCT 116 cells labeling CSNK1A1/CSNK1A1L with Rabbit anti-CSNK1A1/CSNK1A1L antibody (NBP3-33146) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CSNK1A1/CSNK1A1L antibody (NBP3-33146) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - Flow cytometric analysis of HCT 116 cells labeling CSNK1A1/CSNK1A1L. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33146, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33146_rabbit-csnk1a1-csnk1a1l-mab-psh04-58-392024884665.jpg "Flow Cytometry: CSNK1A1/CSNK1A1L Antibody (PSH04-58) [NBP3-33146] - Flow cytometric analysis of HCT 116 cells labeling CSNK1A1/CSNK1A1L. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33146, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")