CRTH-2/GPR44 Antibody (54N3F8)

Images

 
Western Blot: GPR44 Antibody (54N3F8) [NBP2-19014] - Analysis of recombinant CRTH2 (GPR44) probed with CRTH2 antibody at 1 ug/ml.
Immunohistochemistry-Paraffin: GPR44 Antibody (54N3F8) [NBP2-19014] - Formalin-fixed, paraffin-embedded human testis stained with CRTH2 antibody at 5 ug/ml using peroxidase-conjugate and DAB chromogen.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clone
54N3F8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CRTH-2/GPR44 Antibody (54N3F8) Summary

Immunogen
A recombinant protein containing multiple external domains of GPR44 (CRTH2) was used as immunogen for the antibody. NP_004769
Specificity
GPR44, or G-protein coupled receptor 44, is also designated as CRTH2 and CD294. GPR44 is expressed specifically on CD4+ Th2 cells and type 2 cytotoxic effector cells
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PTGDR2
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1-5 ug/ml
  • Immunohistochemistry 1:20-1:1000
  • Immunohistochemistry-Paraffin 5 ug/ml
Application Notes
Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CRTH-2/GPR44 Antibody (54N3F8)

  • CD294 antigen
  • CD294
  • Chemoattractant receptor-homologous molecule expressed on Th2 cells
  • CRTH2
  • CRTH-2
  • CRTH2chemoattractant receptor homologous molecule expressed on T helper type 2 cells
  • DL1R
  • DP2
  • G protein-coupled receptor 44
  • GPR44
  • PGD2R
  • PTGDR2
  • putative G-protein coupled receptor 44

Background

GPR44, or G-protein coupled receptor 44, is also designated as CRTH2 and CD294. CRTH2 is expressed specifically on CD4+ Th2 cells and type 2 cytotoxic effector cells (1). CRTH2 is a prostaglandin D2 receptor PGD2 and has some similar functions to other chemokines exerting chemotaxis of Th2 cells as well as eosinophils and basophils (2). Interestingly inhibition of hair growth by PGD2 is through CRTH2 so the thought is that inhibition of CRTH2 may also eliminate hair-loss or balding effects of PGD2. This implies that inhibitors to CRTH2 could be the new Rogaine(?) (3, 4). CRTH2 is thought to be important in allergic diseases, including asthma, and several companies are already testing drugs that block CRTH2 in clinical trials to treat these diseases (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-19014AF405
Alexa Fluor 488 Labeled NBP2-19014AF488
Alexa Fluor 647 Labeled NBP2-19014AF647
Alexa Fluor 700 Labeled NBP2-19014AF700
Biotin Labeled NBP2-19014B
DyLight 350 Labeled NBP2-19014UV
DyLight 405 Labeled NBP2-19014V
DyLight 488 Labeled NBP2-19014G
DyLight 550 Labeled NBP2-19014R
DyLight 650 Labeled NBP2-19014C
DyLight 680 Labeled NBP2-19014FR
DyLight 755 Labeled NBP2-19014IR
HRP Labeled NBP2-19014H

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Bioinformatics

Gene Symbol PTGDR2
Entrez