CRTH-2/GPR44 Antibody (54N1H4)

Western Blot: GPR44 Antibody (54N1H4) [NBP2-19013] - Analysis of recombinant GPR44 (CRTH2) probed with GPR44 antibody at 1 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

• Reactivity: Hu
• Applications: WB
• Clone: 54N1H4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CRTH-2/GPR44 Antibody (54N1H4) Summary

• Immunogen: A recombinant protein containing multiple external domains of GPR44 (CRTH2) was used as immunogen for the antibody. NP_004769
• Specificity: GPR44, or G-protein coupled receptor 44, is also designated as CRTH2 and CD294. GPR44 is expressed specifically on CD4+ Th2 cells and type 2 cytotoxic effector cells
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTGDR2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-5 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for CRTH-2/GPR44 Antibody (54N1H4)

• CD294 antigen
• CD294
• Chemoattractant receptor-homologous molecule expressed on Th2 cells
• CRTH2
• CRTH-2
• CRTH2chemoattractant receptor homologous molecule expressed on T helper type 2 cells
• DL1R
• DP2
• G protein-coupled receptor 44
• GPR44
• PGD2R
• PTGDR2
• putative G-protein coupled receptor 44

Background

GPR44, or G-protein coupled receptor 44, is also designated as CRTH2 and CD294. GPR44 is expressed specifically on CD4+ Th2 cells and type 2 cytotoxic effector cells (1). GPR44 is a prostaglandin D2 receptor PGD2 and has some similar functions to other chemokines exerting chemotaxis of Th2 cells as well as eosinophils and basophils (2). Interestingly inhibition of hair growth by PGD2 is through GPR44 so the thought is that inhibition of GPR44 may also eliminate hair-loss or balding effects of PGD2 . This implies that inhibitors to GPR44 could be the new Rogaine(?) (3, 4). GPR44 is thought to be important in allergic diseases, including asthma, and several companies are already testing drugs that block GPR44 in clinical trials to treat these diseases (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTGDR2