CRTAM Antibody (1C11E10) - BSA Free

Flow Cytometry: CRTAM Antibody (1C11E10) [NBP3-27154] - Analysis of HL-60 cells using CRTAM mouse mAb (green) and negative control (red).

Western Blot: CRTAM Antibody (1C11E10) [NBP3-27154] - Analysis using CRTAM mAb against HEK293 (1) and CRTAM (AA: extra 18-287)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: CRTAM Antibody (1C11E10) [NBP3-27154] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Western Blot: CRTAM Antibody (1C11E10) [NBP3-27154] - Analysis using CRTAM mAb against human CRTAM (AA: extra 18-287) recombinant protein. (Expected MW is 56 kDa)

• Reactivity: Hu
• Applications: WB, ELISA, Flow
• Clone: 1C11E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CRTAM Antibody (1C11E10) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human CRTAM (AA: extra 18-287) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CRTAM
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 44.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for CRTAM Antibody (1C11E10) - BSA Free

• CD355 antigen
• CD355
• class I MHC restricted T cell associated molecule
• class-I MHC-restricted T cell associated molecule
• Class-I MHC-restricted T-cell-associated molecule
• CRTAM
• cytotoxic and regulatory T cell molecule
• cytotoxic and regulatory T-cell molecule

Background

The CRTAM gene is upregulated in CD4 (see MIM 186940)-positive and CD8 (see CD8A; MIM 186910)-positive T cells and encodes a type I transmembrane protein with V and C1-like Ig domains (Yeh et al., 2008 [PubMed 18329370]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CRTAM