Crry Antibody (TLD-1C11) - Azide Free

• Reactivity: Rt
• Applications: WB, ELISA, Flow, Func, ICC/IF, IHC
• Clone: TLD-1C11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide Free
• Concentration: 0.1 mg/ml

Crry Antibody (TLD-1C11) - Azide Free Summary

• Immunogen: Rat microglial cells
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: Cr1l
• Purity: Protein G purified
• Endotoxin Note: <24 EU/mg

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Functional
  • Immunoassay
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Frozen
  • Western Blot
• Application Notes: The monoclonal antibody can be used to block autoimmune phenomena in rats. Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: No Preservative
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for Crry Antibody (TLD-1C11) - Azide Free

• Complement component (3b/4b) receptor 1-like
• Complement component receptor 1-like protein
• Complement receptor type-1 related gene Y
• Complement regulatory protein Crry
• CR
• Cr1l
• CRY
• MCP
• mCR
• mCRY
• p65
• Protein p65

Background

The monoclonal antibody TLD-1C11 reacts with rat-complement regulator Crry. Complement comprises a system of soluble serum proteins that, upon activiation by antibody (classical pathway), sugars (lectin pathway), or bacterial and foreign surfaces (alternative pathway), directly causes cell damage. Complement is constantly activated at low levels in serum, requiring cells to be protect themselves from damage. In human, cells are protected from complement-mediated damage by expression of membrane-bound complement regulatory proteins, which act at serveral points along the cascade. Human cells are protected by decay-accelerating factor (DAF, CD55), membrane cofactor protein (MCP, CD46) and membrane attack complex inhibitor protein 1 (MIN1, CD59). Furthermore the rat analogues of DAF, MCP and CD59 have been characterized. Rats and mice express a unique membrane complement regulator of the activating pathways, called complement receptor type-1 related gene Y (Crry). In rats there are two isoforms of Crry, with six or seven short consensus repeats (SCR), encoding 65-70 and 75-85 kDa proteins, respectively, which are both transmembrane anchored. Although Crry has the functional properties of both DAF and MCP, it does not replace them. All rat blood cells express Crry whereas approximately 37 and 39% of the rat T cells express respectively DAF and CD59. Studies in rat revealed that despite lacking DAF and CD59, these double-negative rat T cells were not more susceptible to homologous complement lysis than cells that expressed both DAF and CD59. However, blocking of the only other known rat complement regulator, Crry, enhanced lysis only at the double-negative cells. In rats, Crry exerts powerful control in the activation pathways where it acts both as a cofactor and as a decay accelerator to inactivate convertases. Crry has been succesfully expressed and refolded in bacteria. The refolded protein has full-complement regulatory activity in vitro, and has been applied in rat models of arthritis and other inflammatory diseases. The monoclonal antibody TLD-1C11 can be used to block autoimmune phenomena in rats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Cr1l