CRMP1 Antibody (JU73-30)

Western Blot: CRMP1 Antibody (JU73-30) [NBP2-75449] - Analysis of CRMP1 on mouse brain tissue lysate using anti-CRMP1 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: CRMP1 Antibody (JU73-30) [NBP2-75449] - Staining CRMP1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CRMP1 Antibody (JU73-30) [NBP2-75449] - Analysis of paraffin-embedded rat brain tissue using anti-CRMP1 antibody. Counter stained with hematoxylin.

Flow Cytometry: CRMP1 Antibody (JU73-30) [NBP2-75449] - Analysis of SH-SY-5Y cells with CRMP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: CRMP1 Antibody (JU73-30) [NBP2-75449] - Staining CRMP1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: CRMP1 Antibody (JU73-30) [NBP2-75449] - Staining CRMP1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CRMP1 Antibody (JU73-30) [NBP2-75449] - Analysis of paraffin-embedded mouse brain tissue using anti-CRMP1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CRMP1 Antibody (JU73-30) [NBP2-75449] - Analysis of paraffin-embedded mouse cerebellum tissue using anti-CRMP1 antibody. Counter stained with hematoxylin.

Product Discontinued

CRMP1 Antibody (JU73-30) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human CRMP1 aa 489-538 / 572. (SwissProt: Q14194 Human; SwissProt: P97427 Mouse; SwissProt: Q62950 Rat)
• Localization: Cytoplasm. Cytoskeleton.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CRMP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000
• Theoretical MW: 62 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CRMP1 Antibody (JU73-30)

• collapsin response mediator protein 1DRP1
• CRMP-1
• dihydropyrimidinase-like 1
• dihydropyrimidinase-related protein 1
• DPYSL1DRP-1dihydropyrimidinase related protein-1
• ULIP3
• ULIP-3
• Unc-33-like phosphoprotein 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRMP1 Antibody (NBP2-75449)(1)

Publication using NBP2-75449

• Brahma MM, Takahashi K, Namekata K et al. Genetic inhibition of collapsin response mediator protein-2 phosphorylation ameliorates retinal ganglion cell death in normal-tension glaucoma models Genes to cells : devoted to molecular & cellular mechanisms 2022-06-15 [PMID: 35703119]

Bioinformatics

• Gene Symbol: CRMP1