Human CRISP-2 ELISA Kit (Colorimetric)

ELISA: Human CRISP-2 ELISA Kit (Colorimetric) [NBP3-27441] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Tissue homogenates and other biological fluids
• Standard Curve Range: 0.156 - 10 ng/mL (example only; lot dependent)
• Sensitivity: 0.055 ng/mL (example only; lot dependent)

Human CRISP-2 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 4.5 hours
• Standard Curve Range: 0.156 - 10 ng/mL (example only; lot dependent)
• Sensitivity: 0.055 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CRISP2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human CRISP-2 ELISA Kit (Colorimetric)

• Cancer/testis antigen 36
• CRISP2
• CRISP-2
• CRISP-2GAPDL5
• CT36TSP1
• cysteine-rich secretory protein 2
• GAPDL5
• glyceraldehyde-3-phosphate dehydrogenase-like 5
• testis specific protein 1 (probe H4-1 p3-1)
• Testis-specific protein TPX-1
• TPX1
• TPX1MGC111136
• TSP1

Background

CRISP2, or Cysteine-rich secretory protein 2, consists of a 243 amino acid isoform that is 27 kDa, and is involved in the regulation of calcium fluxation and ion channel activity during sperm capacitation. Current research is being conducted on CRISP2 and its relation to several diseases and disorders, including male infertility, atopic dermatitis, and sinusitis. The protein is not currently linked to any specific pathways or biological processes, but it interacts with PPP2R1A and PPP2R1B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for CRISP-2 ELISA Kit (NBP3-27441). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CRISP2