CRIPTO Antibody

Immunohistochemistry-Paraffin: CRIPTO Antibody [NBP1-68841] - Cripto1 Antibody [NBP1-68841] (3.8ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CRIPTO Antibody Summary

• Description: Novus Biologicals Goat CRIPTO Antibody (NBP1-68841) is a polyclonal antibody validated for use in IHC and ELISA. Anti-CRIPTO Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence YGRNCEHDVRKEN corresponding to internal region according to NP_003203.1.
• Epitope: YGRNCEHDVRKEN
• Marker: Embryonic Stem Cell Marker
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CRIPTO
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:128000
• Application Notes: IHC-P: Human placenta shows stainng assocated with nuclei in some trophoblasts and weak cytoplasm staining in cytotrophoblasts.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CRIPTO Antibody

• CFC-2
• CR
• Cripto
• Cripto-1 growth factor
• Cripto-1
• CRIPTOCRGF
• Epidermal growth factor-like cripto protein CR1
• TDGF1
• TDGF-1
• teratocarcinoma-derived growth factor 1

Background

The cripto gene is expressed both in ES cells and during the early phases of embryo development, while, in the adult, it is reactivated in a wide range of epithelial cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRIPTO Antibody (NBP1-68841)(2)

Publications using NBP1-68841

• Lee HY, Jung JH, Cho HM et al. GRP78 Protein Expression as Prognostic Values in Neoadjuvant Chemoradiotherapy and Laparoscopic Surgery for Locally Advanced Rectal Cancer Cancer Res Treat. 2015-01-30 [PMID: 25687871] (IHC-P, Human)
• Parisi S, D'Andrea D, Lago CT et al. Nodal-dependent Cripto signaling promotes cardiomyogenesis and redirects the neural fate of embryonic stem cells J Cell Biol 2003-10-27 [PMID: 14581455]

Review for CRIPTO Antibody (NBP1-68841) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC Mouse 06/09/2017

Summary

• Application: Immunohistochemistry
• Sample Tested: 8
• Species: Mouse
• Lot: n/a

Comments

• Comments: traditional protocol without unmasking steps (as recommended for human samples)

FAQs for CRIPTO Antibody (NBP1-68841). (Showing 1 - 1 of 1 FAQs).

1. Our customer want to know the positive control of: NB100-91794 and NBP1-68841. Could you let me know about it?
   • In regards to NB100-91794, positive controls would be Cos7 cells for WB or Human breast carcinoma tissue for IHC. For NBP1-68841, the positive control is human placenta for IHC.

Bioinformatics

• Gene Symbol: CRIPTO
• Entrez:
  • 6997(Human)
  • 21667(Mouse)
  • 680246(Rat)
• Uniprot:
  • NP_003203.1(Human)