NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." alt="Cripto-1 was detected in immersion fixed human induced pluripotent stem cells (iPSCs) using Mouse Anti-Human/Primate Cripto-1 Monoclonal Antibody (Catalog # MAB2772) at 20 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 1538-CR-010/CF | |||
NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell secretion. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." alt="Cripto was detected in immersion fixed D3 mouse embryonic stem cell line using Sheep Anti-Mouse Cripto Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1538) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell secretion. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1538-SP | |||
NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell secretion. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." alt="Cripto was detected in immersion fixed D3 mouse embryonic stem cell line using Rat Anti-Mouse Cripto Monoclonal Antibody (Catalog # MAB1538) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell secretion. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB1538A | |||
| Carrier Free | MAB1538-SP | |||
| Carrier Free | FAB1538V-100UG | |||
| Carrier Free | FAB1538U-100UG | |||
| Carrier Free | FAB1538T-100UG | |||
| Carrier Free | FAB1538S-100UG | |||
| Carrier Free | FAB1538R-100UG | |||
| Carrier Free | FAB1538N-100UG | |||
| Carrier Free | FAB1538G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF145-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 145-CR-010/CF | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB15381-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22065-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21281-25ul | |||
MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # 
MAB002, open histogram) followed by anti-Mouse IgG APC-conjugated Secondary Antibody (
145-CR) was serially diluted and captured by Mouse Anti-Human/Primate Cripto 1 Monoclonal Antibody (Catalog # MAB2772) coated on a Clear Polystyrene Microplate (Catalog # ![N/A CRIPTO [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Cripto_DY145_ELISA_1650.jpg "N/A CRIPTO [Biotin]")
![Immunohistochemistry-Paraffin: CRIPTO Antibody [NBP1-68841] - Cripto1 Antibody [NBP1-68841] (3.8ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/CRIPTO-Antibody-Immunohistochemistry-Paraffin-NBP1-68841-img0002.jpg "Immunohistochemistry-Paraffin: CRIPTO Antibody [NBP1-68841] - Cripto1 Antibody [NBP1-68841] (3.8ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/ Immunofluorescence: CRIPTO Antibody (JE33-31) [NBP3-32221] - Immunocytochemistry analysis of NCCIT cells labeling CRIPTO with Rabbit anti-CRIPTO antibody (NBP3-32221) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CRIPTO antibody (NBP3-32221) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32221_rabbit-cripto-mab-je33-31-2920241114828.jpg "Immunocytochemistry/ Immunofluorescence: CRIPTO Antibody (JE33-31) [NBP3-32221] - Immunocytochemistry analysis of NCCIT cells labeling CRIPTO with Rabbit anti-CRIPTO antibody (NBP3-32221) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CRIPTO antibody (NBP3-32221) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: CRIPTO Antibody (JE33-31) [NBP3-32221] - Flow cytometric analysis of NCCIT cells labeling CRIPTO.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32221, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32221_rabbit-cripto-mab-je33-31-392024884631.jpg)
![Western blot analysis of NCCIT cell lysates with NBP3-32221 at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32221 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32221_-western-blot-639204595238446228.jpg)
![Western Blot: CRIPTO Antibody (SR1320) [NBP3-22065] - Western blot analysis using NBP3-22065 in NCCIT cell lysate.](http://images.novusbio.com/fullsize/nbp3-22065_rabbit-cripto-mab-sr1320-572023156474.jpg "Western Blot: CRIPTO Antibody (SR1320) [NBP3-22065] - Western blot analysis using NBP3-22065 in NCCIT cell lysate.")
