CRIPTO Antibody [DyLight 488]

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Product Details

Summary
Reactivity Hu, Mu, Bv, ZeSpecies Glossary
Applications WB, Flow, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 488

CRIPTO Antibody [DyLight 488] Summary

Immunogen
A synthetic peptide made to an internal portion of human Cripto1 (between residues 100-150). [UniProt# P13385]
Localization
Membrane-bound
Marker
Embryonic Stem Cell Marker
Predicted Species
Mouse (93%), Zebrafish (93%), Bovine (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TDGF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
Application Notes
This Cripto1 antibody is useful in Immunocytochemistry/Immunofluorescence, Flow Cytometry, and Western blot, In Western blot a band is seen ~18 kDa which is the core form of Cripto1. In ICC/IF, cytoplasmic and membrane staining was observed in HeLa cells.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen has 93% homology with mouse, cow, and Zebrafish protein. There is no significant homology with any other species.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CRIPTO Antibody [DyLight 488]

  • CFC-2
  • CR
  • Cripto
  • Cripto-1 growth factor
  • Cripto-1
  • CRIPTOCRGF
  • Epidermal growth factor-like cripto protein CR1
  • TDGF1
  • TDGF-1
  • teratocarcinoma-derived growth factor 1

Background

The cripto gene is expressed both in ES cells and during the early phases of embryo development, while, in the adult, it is reactivated in a wide range of epithelial cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TDGF1
Entrez