CRIM1 Antibody

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

CRIM1 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CRIM1
  Leu35-Ser939
  Accession # Q9NZV1
• Specificity: Detects human CRIM1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CRIM1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CRIM1 Antibody

• CRIM1
• CRIM-1
• cysteine rich transmembrane BMP regulator 1 (chordin-like)
• cysteine-rich motor neuron 1 protein
• cysteine-rich motor neuron 1
• Cysteine-rich repeat-containing protein S52
• MGC138194
• S52

Background

Cysteine rich motor neuron 1 (CRIM1) is a type I transmembrane glycoprotein of the chordin-like cysteine-rich repeat (CRR) family of BMP inhibitors (1‑4). The ~130 kDa, 1036 amino acid (aa) CRIM1 contains a 34 aa signal sequence, a 905 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 76 aa cytoplasmic domain. The ECD includes an N-terminal IGF-binding protein-like motif and six chordin-like von Willebrand C-type CRRs. The ECD can be released from the cell, presumably by proteolytic processing (4). Human CRIM1 ECD shows 88%, 88%, 91%, 86%, 87%, 83% and 72% aa identity with mouse, rat, dog, cow, opossum, chick and zebrafish CRIM1 ECD, respectively. CRIM1 can interact with TGF-beta family ligands, including BMPs 2, 4 and 7, via its CRR domains (4). It binds BMPs intracellularly and antagonizes them by lowering their expression, processing and secretion (4). CRIM1 is expressed in the developing spinal cord in the floor plate and developing motor neurons (1). It is also expressed by perivascular smooth muscle cells and aligns at points of cell-cell contact during endothelial cell capillary formation (2). Endothelial cell expression in vitro appears to be specific to cells that are adherent and growing (2). CRIM1 is also expressed in a spatially and temporally restricted manner in the developing lens, limbs, kidney, teeth and testis (5). Studies where CRIM1 expression is manipulated in developing mouse, chick and zebrafish support its involvement in regulation of vascular and somitic development and organogenesis (5‑7).

1. Kolle, G. et al. (2000), Mech. Dev. 90:181.
2. Glienke, J. et al. (2002) Mech. Dev. 119:165.
3. Abreu, J. G. et al. (2002) Gene 287:39.
4. Wilkinson, L. et al. (2003) J. Biol. Chem. 278:34181.
5. Pennisi, D. J. et al. (2007) Dev. Dyn. 236:502.
6. Kolle, G. et al. (2003) Dev. Dyn. 226:107.
7. Kinna, G. et al. (2006) Mech. Dev. 123:277.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRIM1 Antibody (AF1917)(2)

Publications using AF1917

• Zhang Y, Wang C Nephroblastoma overexpressed (NOV/CCN3) gene: a paired-domain-specific PAX3-FKHR transcription target that promotes survival and motility in alveolar rhabdomyosarcoma cells. Oncogene, 2011-03-21;30(32):3549-62. 3/21/2011 [PMID: 21423212]
• Butler GS, Dean RA, Tam EM, Overall CM Pharmacoproteomics of a metalloproteinase hydroxamate inhibitor in breast cancer cells: dynamics of membrane type 1 matrix metalloproteinase-mediated membrane protein shedding. Mol. Cell. Biol., 2008-05-27;28(15):4896-914. 2008-05-27 [PMID: 18505826] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CRIM1
• Uniprot:
  • Q9NZV1()