CREB5 Overexpression Lysate (Native)

Images

 
Western Blot: CREB5 Overexpression Lysate (Adult Normal) [NBL1-09467] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CREB5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

CREB5 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC209289 and based on accession number NM_001011666. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens cAMP responsive element binding protein 5 (CREB5), transcript variant 4, mRNA.
Gene
CREB5

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for CREB5

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for CREB5 Overexpression Lysate (Native)

  • cAMP response element binding protein CRE-Bpa
  • cAMP response element-binding protein CRE-BPa
  • cAMP responsive element binding protein 5 isoform alpha
  • cAMP responsive element binding protein 5
  • cAMP-responsive element-binding protein 5
  • CREB-5
  • CREBPA
  • CRE-BPA
  • cyclic AMP-responsive element-binding protein 5
  • H_GS165L15.1

Background

The product of this gene belongs to the CRE (cAMP response element)-binding protein family. Members of this family contain zinc-finger and bZIP DNA-binding domains. The encoded protein specifically binds to CRE as a homodimer or a heterodimer with c-Jun or CRE-BP1, and functions as a CRE-dependent trans-activator. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]. Transcript Variant: This variant (4) differs in the 5' UTR and the 5' coding region, compared to variant 1. The resulting isoform (delta) contains a distinct N-terminus, compared to isoform alpha.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CREB5