CREB5 Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC209289 and based on accession number NM_001011666. The protein contains a C-terminal DDK tag.
Homo sapiens cAMP responsive element binding protein 5 (CREB5), transcript variant 4, mRNA.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Lysate Details for CREB5
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for CREB5 Overexpression Lysate (Native)
- cAMP response element binding protein CRE-Bpa
- cAMP response element-binding protein CRE-BPa
- cAMP responsive element binding protein 5 isoform alpha
- cAMP responsive element binding protein 5
- cAMP-responsive element-binding protein 5
- cyclic AMP-responsive element-binding protein 5
The product of this gene belongs to the CRE (cAMP response element)-binding protein family. Members of this family contain zinc-finger and bZIP DNA-binding domains. The encoded protein specifically binds to CRE as a homodimer or a heterodimer with c-Jun or CRE-BP1, and functions as a CRE-dependent trans-activator. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]. Transcript Variant: This variant (4) differs in the 5' UTR and the 5' coding region, compared to variant 1. The resulting isoform (delta) contains a distinct N-terminus, compared to isoform alpha.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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