CREB Antibody (LB9)

Images

 
Western Blot: CREB Antibody (LB9) [NB100-74393] - Analysis of HepG2 cells
Immunocytochemistry: CREB Antibody (LB9) [NB100-74393] - Staining of CREB (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. ...read more
Immunohistochemistry-Paraffin: CREB Antibody (LB9) [NB100-74393] - Normal biopsies of deparaffinized Human tonsil tissue.
Western Blot: CREB Antibody (LB9) [NB100-74393] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing CREB ...read more
Immunocytochemistry: CREB Antibody (LB9) [NB100-74393] - Analysis of CREB (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. ...read more
Immunohistochemistry-Paraffin: CREB Antibody (LB9) [NB100-74393] - Cancer biopsies of deparaffinized human bladder and rectal tissue.
Immunoprecipitation: CREB Antibody (LB9) [NB100-74393] - Analysis of CREB was performed on untreated 293T cells. Antigen: antbody complex was formed by binding 500ug whole cell lysate with 2ug of mouse monoclonal ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, IP
Clone
LB9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CREB Antibody (LB9) Summary

Immunogen
Recombinant human CREB-A protein.
Specificity
CREB (LB9)
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CREB1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 0.1 - 1.0 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 2 ug
Application Notes
WB: Detects an approx. 43 kDa protein.
Positive Control
CREB Lysate (NBL1-09461)
Publications
Read Publication using
NB100-74393 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for CREB Antibody (LB9)

  • active transcription factor CREB
  • cAMP responsive element binding protein 1
  • cAMP-response element-binding protein-1
  • cAMP-responsive element-binding protein 1
  • CREB
  • CREB1
  • CREB-1
  • cyclic AMP-responsive element-binding protein 1
  • MGC9284
  • transactivator protein

Background

Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB appears to play an important role in learning and memory. CREB knock out mice show diminished learning ability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-74393
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP

Publications for CREB Antibody (NB100-74393)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CREB Antibody (NB100-74393). (Showing 1 - 1 of 1 FAQ).

  1. May I know which creb1 antibody is good for ChIP? I found a paper using your rabbit polyclonal creb1 antibody for ChIP but they didn't indicate the catalog number.
    • We are not aware of any of our Creb1 antibodies being used in ChIP at this time. If you can provide us with the PMID of the paper you are referring to I can contact the author. Otherwise, you can use our Innovators Reward Program to try an antibody in a novel species and application.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CREB Antibody (NB100-74393)

Discover related pathways, diseases and genes to CREB Antibody (NB100-74393). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on CREB.

Exploring the Many Roles of PGC-1 alpha
The peroxisome proliferator-activated receptor gamma, co-activator 1 (PGC-1 alpha or PPARGC1A) gene encodes a 91 kDa nuclear protein that acts as a transcriptional co-activator involved in energy metabolism. Interaction with PPAR gamma allows it to in...  Read full blog post.

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Bioinformatics

Gene Symbol CREB1
Entrez