Western Blot: CREB Antibody (LB9) [NB100-74393] - Analysis of HepG2 cells
Immunocytochemistry: CREB Antibody (LB9) [NB100-74393] - Staining of CREB (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. ...read more
Immunohistochemistry-Paraffin: CREB Antibody (LB9) [NB100-74393] - Normal biopsies of deparaffinized Human tonsil tissue.
Western Blot: CREB Antibody (LB9) [NB100-74393] - Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing CREB ...read more
Immunocytochemistry: CREB Antibody (LB9) [NB100-74393] - Analysis of CREB (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. ...read more
Immunohistochemistry-Paraffin: CREB Antibody (LB9) [NB100-74393] - Cancer biopsies of deparaffinized human bladder and rectal tissue.
Immunoprecipitation: CREB Antibody (LB9) [NB100-74393] - Analysis of CREB was performed on untreated 293T cells. Antigen: antbody complex was formed by binding 500ug whole cell lysate with 2ug of mouse monoclonal ...read more
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
PBS with 1 mg/ml BSA
0.05% Sodium Azide
Protein A purified
Alternate Names for CREB Antibody (LB9)
active transcription factor CREB
cAMP responsive element binding protein 1
cAMP-response element-binding protein-1
cAMP-responsive element-binding protein 1
cyclic AMP-responsive element-binding protein 1
Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB appears to play an important role in learning and memory. CREB knock out mice show diminished learning ability.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for CREB Antibody (NB100-74393). (Showing 1 - 1 of 1 FAQ).
May I know which creb1 antibody is good for ChIP? I found a paper using your rabbit polyclonal creb1 antibody for ChIP but they didn't indicate the catalog number.
We are not aware of any of our Creb1 antibodies being used in ChIP at this time. If you can provide us with the PMID of the paper you are referring to I can contact the author. Otherwise, you can use our Innovators Reward Program to try an antibody in a novel species and application.
Exploring the Many Roles of PGC-1 alpha The peroxisome proliferator-activated receptor gamma, co-activator 1 (PGC-1 alpha or PPARGC1A) gene encodes a 91 kDa nuclear protein that acts as a transcriptional co-activator involved in energy metabolism. Interaction with PPAR gamma allows it to in... Read full blog post.
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