CRACC/SLAMF7 Antibody (235614) [Unconjugated]

MAB003) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). View our protocol for Staining Membrane-associated Proteins." > MAB003) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). View our protocol for Staining Membrane-associated Proteins." title="Human PBMCs were stained with either (A) Mouse Anti-Human CRACC/SLAMF7 Monoclonal Antibody (MAB1906) or (B) Mouse IgG2A Isotype Control (MAB003) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B). View our protocol for Staining Membrane-associated Proteins." />

Human PBMCs were stained with either (A) Mouse Anti-Human CRACC/SLAMF7 Monoclonal Antibody (MAB1906) or (B) Mouse IgG2A Isotype Control (<a class=

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 235614
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CRACC/SLAMF7 Antibody (235614) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CRACC/SLAMF7
  Lys27-Ser225
  Accession # Q9NQ25
• Specificity: Detects human CRACC/SLAMF7 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human NTB-AR is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SLAMF7
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CRACC/SLAMF7 Antibody (235614) [Unconjugated]

• 19A
• CD2 subset 1
• CD2-like receptor activating cytotoxic cells
• CD319 antigen
• CD319
• CRACC
• CRACCCD2-like receptor-activating cytotoxic cells
• CS119A24 protein
• Membrane protein FOAP-12
• novel LY9 (lymphocyte antigen 9) like protein
• Novel Ly9
• Protein 19A
• SLAM family member 7
• SLAMF7

Background

CRACC, also known as CSI (CD2 subset I), is a type I transmembrane protein belonging to the CD2 subset of the Ig superfamily. CRACC is expressed on most NK cells and subsets of CD8⁺ cells, CD4⁺ cells and B cells. CRACC may play a role in the activation and effector function of T cells and NK cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRACC/SLAMF7 Antibody (MAB1906)(1)

Publication using MAB1906

• Hislop AD, Palendira U, Leese AM Impaired Epstein-Barr virus-specific CD8+ T-cell function in X-linked lymphoproliferative disease is restricted to SLAM family-positive B-cell targets. Blood, 2010-07-19;116(17):3249-57. 2010-07-19 [PMID: 20644117] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: SLAMF7
• Uniprot:
  • Q9NQ25()