CPSF73 Antibody

Western Blot: CPSF73 Antibody [NB100-57529] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. NB100-57529 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B) and used for IP at 3 ug/mg lysate.

Immunohistochemistry-Paraffin: CPSF73 Antibody [NB100-57529] - Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-CPSF73 used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu, Bv
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CPSF73 Antibody Summary

• Description: Novus Biologicals Rabbit CPSF73 Antibody (NB100-57529) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 634 and 684 of human cleavage and polyadenylation specific factor 3, 73kDa using the numbering given in entry NP_057291.1 (GeneID 51692).
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CPSF3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 mcg/mg
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CPSF73 Antibody

• cleavage and polyadenylation specific factor 3, 73kD subunit
• cleavage and polyadenylation specific factor 3, 73kDa
• cleavage and polyadenylation specificity factor 3
• Cleavage and polyadenylation specificity factor 73 kDa subunit
• cleavage and polyadenylation specificity factor subunit 3
• CPSF 73 kDa subunit
• CPSF3
• CPSF73
• CPSF-73
• CPSF73CPSF
• EC 3.1.27
• EC 3.1.27.-
• mRNA 3'-end-processing endonuclease CPSF-73
• YSH1

Background

CPSF73 is the 73 kDa subunit of the cleavage and polyadenylation specificity factor (CPSF) complex that functions in pre-mRNA-3'-end formation. CPSF73 has been implicated as the endonuclease component of the CPSF complex for pre-mRNA-3' end processing. CPSF73 is also known as cleavage and polyadenylation specificity factor subunit 3, cleavage and polyadenylation specificity factor 73 kDa subunit, CPSF 73 kDa subunit, and CPSF-73.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CPSF3
• Entrez:
  • 51692(Human)
  • 54451(Mouse)
• Uniprot:
  • NP_057291.1(Human)