CPSF2 Antibody - BSA Free

Western Blot: CPSF2 Antibody [NBP1-82910] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

Immunocytochemistry/Immunofluorescence: CPSF2 Antibody [NBP1-82910] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

Immunohistochemistry-Paraffin: CPSF2 Antibody [NBP1-82910] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.

Western Blot: CPSF2 Antibody [NBP1-82910] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CPSF2 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: IELRKRVKLEGKELEEYLEKEKLKKEAAKKLEQSKEADIDSSDESDIEEDIDQPSAHKTKHDLMMKGEGSRKGSFFKQAKKSYPMFPAPEERIKWDEYGEIIKPEDFLVPELQATEEEKSKLESGLTNGDEPMDQDLSDVPTKCISTTESI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CPSF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  CPSF2 Protein (NBP1-82910PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CPSF2 Antibody - BSA Free

• cleavage and polyadenylation specific factor 2, 100kDa
• Cleavage and polyadenylation specificity factor 100 kDa subunit
• cleavage and polyadenylation specificity factor subunit 2
• CPSF 100 kDa subunit
• CPSF 100kDa subunit
• CPSF100
• KIAA1367cleavage and polyadenylation specific factor 2, 100kD subunit

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CPSF2 Antibody (NBP1-82910)(1)

Publication using NBP1-82910

• Kolev NG, Yario TA, Benson E et al. Conserved motifs in both CPSF73 and CPSF100 are required to assemble the active endonuclease for histone mRNA 3'-end maturation. EMBO Rep 2008-10-01 [PMID: 18688255]

Bioinformatics

• Gene Symbol: CPSF2