CPSF1 Antibody

Western Blot: CPSF1 Antibody [NB100-79822] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-CPSF160 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescencewith an exposure time of 30 seconds.

Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CPSF160 (NB100-79822). Detection: DAB

Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-CPSF160 (NB100-79822). Detection: DAB

Immunoprecipitation: CPSF1 Antibody [NB100-79822] - Detection of human CPSF160 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CPSF160 antibody NB100-79822 (lot NB100-79822-2) used for IP at 6 ug per reaction. CPSF160 was also immunoprecipitated by a previous lot of this antibody (lot NB100-79822-1). For blotting immunoprecipitated CPSF160, NB100-79822 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Mu, Bv
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CPSF1 Antibody Summary

• Description: Novus Biologicals Rabbit CPSF1 Antibody (NB100-79822) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1392 and 1442 of human cleavage and polyadenylation specific factor 1, 160kD subunit using the numbering given in entry NP_037423.1 (GeneID 29894).
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CPSF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CPSF1 Antibody

• cleavage and polyadenylation specific factor 1, 160kD subunit
• cleavage and polyadenylation specific factor 1, 160kDa
• Cleavage and polyadenylation specificity factor 160 kDa subunit
• cleavage and polyadenylation specificity factor subunit 1
• CPSF 160 kDa subunit
• CPSF160
• HSU37012
• P/cl.18
• polyadenylation specificity factor

Background

Cleavage and polyadenylation specificity factor (CPSF) is a multisubunit complex that plays a central role in 3-prime processing of pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis. CPSF1 is the largest subunit of the CPSF complex (Murthy and Manley, 1995 [PubMed 7590244]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CPSF1
• Entrez:
  • 29894(Human)
  • 94230(Mouse)
• Uniprot:
  • Q10570(Human)