CPSF1 Antibody

Images

 
Western Blot: CPSF1 Antibody [NB100-79822] - Detection of Human and Mouse CPSF160 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP ...read more
Immunocytochemistry/ Immunofluorescence: CPSF1 Antibody [NB100-79822] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CPSF160 used at a dilution of 1:100. Detection: Red-fluorescent ...read more
Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822] - Human ovarian carcinoma (left) and mouse renal cell carcinoma (right). Antibody used at a dilution of 1:200 (1ug/ml).
Proximity Ligation Assay: CPSF1 Antibody [NB100-79822] - Secondary-conjugate Duolink In Situ PLA in MCF-7 cells. goat anti-human MCM2 (NB100-244) and rabbit anti-human CPSF160 (NB100-79822). Image merged from DAPI ...read more
Proximity Ligation Assay: CPSF1 Antibody [NB100-79822] - Secondary-conjugate Duolink In Situ PLA in MCF-7 cells. goat anti-human POLR2A (NBP2-14859) and rabbit anti-human CPSF160 (NB100-79822). Image merged from DAPI ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

CPSF1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1392 and 1442 of human cleavage and polyadenylation specific factor 1, 160kD subunit using the numbering given in entry NP_037423.1 (GeneID 29894).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CPSF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:50 - 1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Proximity Ligation Assay 1:200- 1:4000

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CPSF1 Antibody

  • cleavage and polyadenylation specific factor 1, 160kD subunit
  • cleavage and polyadenylation specific factor 1, 160kDa
  • Cleavage and polyadenylation specificity factor 160 kDa subunit
  • cleavage and polyadenylation specificity factor subunit 1
  • CPSF 160 kDa subunit
  • CPSF160
  • HSU37012
  • P/cl.18
  • polyadenylation specificity factor

Background

Cleavage and polyadenylation specificity factor (CPSF) is a multisubunit complex that plays a central role in 3-prime processing of pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis. CPSF1 is the largest subunit of the CPSF complex (Murthy and Manley, 1995 [PubMed 7590244]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CPSF1
Entrez
Uniprot