CPN1 Antibody

HepG2 whole cell extract and conditioned medium (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CPN1 antibody [N1C3] (NBP3-48455) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CPN1 Antibody Summary

• Description: Novus Biologicals Rabbit CPN1 Antibody (NBP3-48455) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human CPN1. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CPN1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for CPN1 Antibody

• ACBP
• Anaphylatoxin inactivator
• Arginine carboxypeptidase
• carboxypeptidase N polypeptide 1 50 kD
• Carboxypeptidase N polypeptide 1
• Carboxypeptidase N small subunit
• carboxypeptidase N, polypeptide 1
• carboxypeptidase N, polypeptide 1, 50kD
• CPN
• CPN1
• CPN-1
• CPNcarboxypeptidase N catalytic chain
• EC 3.4.17
• EC 3.4.17.3
• FLJ40792
• kininase I
• kininase-1
• Lysine carboxypeptidase
• Plasma carboxypeptidase B
• SCPN
• SCPNcarboxypeptidase N catalytic subunit
• Serum carboxypeptidase N

Background

Carboxypeptidase N (arginine carboxypeptidase or CPN) cleaves basic amino acid residues from the carboxy terminal of peptides and proteins. The enzyme plays a central role in regulating the biologic activity of peptides such as kinins and anaphylatoxins, and therefore is also known as kininase-1 and anaphylatoxin inactivator. CPN is a tetrameric complex consisting of two identical regulatory subunits (CPN reg) and two identical catalytic subunits (CPN cat). The two glycosylated CPN reg subunits protect the two CPN cat subunits and keep them in the circulation. CPN reg is a member of the leucinerich repeat family of proteins and the gene which encodes CPN reg maps to human chromosome 8p22-p23. CPN cat is a member of the regulatory B-type carboxypeptidase group and the gene which encodes CPN cat maps to human chromosome 10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CPN1