COX4-I1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of human brain tissue, Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and BaF3 mouse pro‑B cell line. PVDF membrane was probed with 1 µg/mL of ...read more
COX4-I1 was detected in immersion fixed paraffin-embedded sections of normal human kidney using Goat Anti-Human/Mouse COX4-I1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5814) at 10 µg/mL overnight at 4 ...read more
COX4‑I1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse COX4‑I1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5814) at 5 µg/mL for 3 hours ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for COX4‑I1 at approximately ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

COX4-I1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human COX4-I1
Ala23-Lys169
Accession # P13073
Specificity
Detects human and mouse COX-4I1 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
COX4I1
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF5814 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for COX4-I1 Antibody [Unconjugated]

  • COX IV-1
  • COX4I1
  • COX4-I1

Background

Cytochrome c oxidase subunit 4 isoform 1 (COX-4I1) is a 21‑22 kDa member of the cytochrome c oxidase IV family of proteins. It is a component of COX, an inner mitochondrial membrane multimeric dimer that catalyzes the transfer of electrons from cytochrome c to dioxygen. COX-4I1 is the largest of 10 distinct nuclear DNA‑encoded subunits that form each COX monomer. Human COX4-I1 is 169 amino acids (aa) in length and possesses a mitochondrial transit peptide between aa 1‑22, an ATP binding site (aa 42; 95‑100), and multiple subunit interface sequences. The ATP binding site is suggested to make COX-4I1 a regulatory subunit within the COX complex. There are two potential splice variants for COX-4I1 that show a three aa substitution for aa 81‑169, and a single Gly substitution for aa 125‑169. COX‑4I2, the product of a different gene, shares only 50% aa identity with COX-4I1. Over amino acid 23‑169, human COX4‑1 shares 78% aa identity with mouse COX‑4I1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for COX4-I1 Antibody (AF5814) (0)

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Review for COX4-I1 Antibody (AF5814) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human and Mouse.

Reviews using AF5814:
Filter by Applications
IF
(1)
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Filter by Species
Human and Mouse
(1)
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Images Ratings Applications Species Date Details
Immunofluorescence COX4-I1 AF5814
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reviewed by:
Anonymous
IF Human and Mouse 09/18/2018
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Summary

ApplicationImmunofluorescence
Sample TestedNSC-34 cells
SpeciesHuman and Mouse
LotCDBS021807C

Comments

CommentsPBS 0.01% Triton 0.3% Fish Skin Gelatin

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WB Video Protocol

FAQs for COX4-I1 Antibody (AF5814) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Array Products

Array AF5814

Bioinformatics Tool for COX4-I1 Antibody (AF5814)

Discover related pathways, diseases and genes to COX4-I1 Antibody (AF5814). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

4
5
0
4
1
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0
2
0
1
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Anonymous
09/18/2018
Application: IF
Species: Human and Mouse

Bioinformatics

Gene Symbol COX4I1
Uniprot