COX4-I1 Antibody [Alexa Fluor® 700]

• Reactivity: Hu, Mu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 700

COX4-I1 Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human COX4-I1
  Ala23-Lys169
  Accession # P13073
• Specificity: Detects human and mouse COX-4I1 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for COX4-I1 Antibody [Alexa Fluor® 700]

• COX IV-1
• COX4
• COX4-1
• COX4I1
• COX4-I1
• COXIV
• COXIV-
• Cytochrome c oxidase polypeptide IV
• cytochrome c oxidase subunit 4 isoform 1, mitochondrial
• cytochrome c oxidase subunit IV
• MC4DN16

Background

Cytochrome c oxidase subunit 4 isoform 1 (COX-4I1) is a 21‑22 kDa member of the cytochrome c oxidase IV family of proteins. It is a component of COX, an inner mitochondrial membrane multimeric dimer that catalyzes the transfer of electrons from cytochrome c to dioxygen. COX-4I1 is the largest of 10 distinct nuclear DNA‑encoded subunits that form each COX monomer. Human COX4-I1 is 169 amino acids (aa) in length and possesses a mitochondrial transit peptide between aa 1‑22, an ATP binding site (aa 42; 95‑100), and multiple subunit interface sequences. The ATP binding site is suggested to make COX-4I1 a regulatory subunit within the COX complex. There are two potential splice variants for COX-4I1 that show a three aa substitution for aa 81‑169, and a single Gly substitution for aa 125‑169. COX‑4I2, the product of a different gene, shares only 50% aa identity with COX-4I1. Over amino acid 23‑169, human COX4‑1 shares 78% aa identity with mouse COX‑4I1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.