COX4 Antibody

Images

 
Western Blot: COX4 Antibody [NBP2-15975] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody. HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: COX IV isoform 1 Antibody [NBP2-15975] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using COX IV antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are ...read more
Immunohistochemistry-Paraffin: COX IV isoform 1 Antibody [NBP2-15975] - Immunohistochemical analysis of paraffin-embedded HS578T xenograft, using antibody at 1:250 dilution.
Western Blot: COX IV isoform 1 Antibody [NBP2-15975] - Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE gel, diluted at 1:1000.
Western Blot: COX IV isoform 1 Antibody [NBP2-15975] - Sample (50 ug of whole cell lysate) A: Mouse Muscle, 12% SDS PAGE gel, diluted at 1:500.
Western Blot: COX IV isoform 1 Antibody [NBP2-15975] - Sample (50 ug of whole cell lysate) A: rat muscle, 12% SDS PAGE gel, diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.83 mg/ml
     

Genetic Strategies

   

Order Details

COX4 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.
Localization
Mitochondrion inner membrane
Marker
Mitochondria Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COX4I1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Bovine (82%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
1.83 mg/ml
Purity
Immunogen affinity purified

Alternate Names for COX4 Antibody

  • COX IV-1
  • COX4
  • COX4-1
  • COX4COXIV
  • COX4I2
  • Cytochrome c oxidase polypeptide IV
  • cytochrome c oxidase subunit 4 isoform 1, mitochondrial
  • cytochrome c oxidase subunit IV isoform 1cytochrome c oxidase subunit IV
  • MGC72016

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for COX4 Antibody (NBP2-15975) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for COX4 Antibody (NBP2-15975)

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Research Areas for COX4 Antibody (NBP2-15975)

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Blogs on COX4.

The Importance of the COX IV Antibody to Apoptosis Research
COX IV isoform 1 is a nuclear-encoded polypeptide chain of the Cytochrome C Oxidase enzyme, located on the mitochondrial inner membrane. Owing to its widespread distribution in human and mammalian tissues, COX IV antibodies are widely used as loading...  Read full blog post.

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Bioinformatics

Gene Symbol COX4I1
Entrez
Uniprot