Mouse Corticotropin Releasing Factor ELISA Kit (Colorimetric)

ELISA: Mouse Corticotropin Releasing Factor ELISA Kit (Colorimetric) [NBP3-06883] - Samples were spiked with high concentrations of Mouse Corticotropin Releasing Factor and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse Corticotropin Releasing Factor ELISA Kit (Colorimetric) [NBP3-06883] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA, ELISA(Comp)
• Suitable Sample Type: Serum, Plasma, Cell supernatant
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Mouse Corticotropin Releasing Factor ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse Corticotropin Releasing Factor in samples. No significant cross-reactivity or interference between Mouse Corticotropin Releasing Factor and analogues was observed.
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Competitive-ELISA
• Inter-Assay: 5.56
• Intra-Assay: 6.54
• Spike Recovery: 88-108 %
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CRH

Applications/Dilutions

• Dilutions:
  • Competitive ELISA
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Corticotropin Releasing Factor ELISA Kit (Colorimetric)

• corticoliberin
• corticotropin releasing hormone
• Corticotropin-releasing hormone
• CRFCorticotropin-releasing factor

Background

Corticotropin-releasing hormone (CRH) is a 41-amino acid peptide derived from a 191-amino acid preprohormone. CRH issecreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in CRH hasbeen observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin dificiency hasmultiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to productionin the hypothalamus, CRH is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed inthe placenta. In the placenta CRH is a marker that determines the length of gestation and the timing of parturitionand delivery. A rapid increase in circulating levels of CRH occurs at the onset of parturition, suggesting that, inaddition to its metabolic functions, CRH may act as a trigger for parturition. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Corticotropin Releasing Factor ELISA Kit (NBP3-06883)(1)

Publication using NBP3-06883

• Cui J, Huang Z, Xie Y et al. Gut microbiota mediated inflammation, neuroendocrine and neurotrophic functions involved in the antidepressant-like effects of diosgenin in chronic restraint stress Journal of Affective Disorders 2022-10-01 [PMID: 36349650] (ELISA)

FAQs for Corticotropin Releasing Factor ELISA Kit (NBP3-06883). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CRH