Corticotropin Releasing Factor Antibody - Azide and BSA Free

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Immunohistochemistry: Corticotropin Releasing Factor Antibody [NB110-81721] - Immunohistochemical detection of Corticotropin Releasing Factor in Rat Hypothalamic Brain Tissue using a 1ug/ml dilution of Affinity Purified ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Gp(-)Species Glossary
Applications ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

Corticotropin Releasing Factor Antibody - Azide and BSA Free Summary

Immunogen
An 18 amino acid synthetic CRF peptide (H-CEQLAQQAHSNRKLMEII-OH), conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity
Specific for Corticotropin Releasing Factor. Confirmed by Immunohistochemical analysis of Paraffin Embedded Rat Brain (Hypothalamus).
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CRH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:10
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Control
Corticotropin Releasing Factor Overexpression Lysate
Publications
Read Publications using
NB110-81721 in the following applications:

Reactivity Notes

This antibody recognises CRF in Human, Rat and Mouse. There is no Cross-reactivity with Guinea Pig.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.05X PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.02 ml sterilized water.

Alternate Names for Corticotropin Releasing Factor Antibody - Azide and BSA Free

  • corticoliberin
  • corticotropin releasing hormone
  • Corticotropin-releasing hormone
  • CRFCorticotropin-releasing factor

Background

Corticotropin-releasing hormone (CRH) is a 41-amino acid peptide derived from a 191-amino acid preprohormone. CRH issecreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in CRH hasbeen observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin dificiency hasmultiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to productionin the hypothalamus, CRH is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed inthe placenta. In the placenta CRH is a marker that determines the length of gestation and the timing of parturitionand delivery. A rapid increase in circulating levels of CRH occurs at the onset of parturition, suggesting that, inaddition to its metabolic functions, CRH may act as a trigger for parturition. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-81721
Species: Hu, Mu, Rt, Gp(-)
Applications: ELISA, ICC/IF, IHC, IHC-P

Publications for Corticotropin Releasing Factor Antibody (NB110-81721)(8)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 4 applications: ELISA, ICC/IF, IHC, IHC-P.


Filter By Application
ELISA
(1)
ICC/IF
(2)
IHC
(3)
IHC-P
(1)
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Filter By Species
Mouse
(2)
Rat
(4)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB110-81721 Applications Species
Wang Y, Zhang Y, Shi Z et al. Exposure of male mice to perfluorooctanoic acid induces anxiety-like behaviors by increasing corticotropin-releasing factor in the basolateral amygdala complex Chemosphere Jan 1 2022 [PMID: 34826932] (IHC, Mouse) IHC Mouse
Nass SR, Lark ARS, Hahn YK et al. HIV-1 Tat and morphine decrease murine inter-male social interactions and associated oxytocin levels in the prefrontal cortex, amygdala, and hypothalamic paraventricular nucleus Hormones and behavior Jun 22 2021 [PMID: 34171549]
Song Y, Meng Q, Wu K et al. Disinhibition of PVN-projecting GABAergic neurons in AV region in BNST participates in visceral hypersensitivity in rats Psychoneuroendocrinology Jan 1 2020 [PMID: 32417623] (IHC, Rat) IHC Rat
Aguiniga LM, Searl TJ, Rahman-Enyart A et al. Acyloxyacyl hydrolase modulates voiding activity Am. J. Physiol. Renal Physiol. Jan 31 2020 [PMID: 32003596]
Aguiniga, LM;Yang, W;Yaggie, RE;Schaeffer, AJ;Klumpp, DJ;, ; ACYLOXYACYL HYDROLASE MODULATES DEPRESSIVE-LIKE BEHAVIORS THROUGH ARYL HYDROCARBON RECEPTOR Am. J. Physiol. Regul. Integr. Comp. Physiol. Apr 24 2019 [PMID: 31017816] (IHC-P, Mouse) IHC-P Mouse
Lee B, Shim I, Lee H et al. Tetramethylpyrazine reverses anxiety-like behaviors in a rat model of post-traumatic stress disorder Korean J Physiol Pharmacol Aug 29 2018 [PMID: 30181699] (ELISA, Rat) ELISA Rat
Zhu J, Chen Z, Meng Z et al. Electroacupuncture Alleviates Surgical Trauma-Induced Hypothalamus Pituitary Adrenal Axis Hyperactivity Via microRNA-142. Front Mol Neurosci. 2017 Oct 12 [PMID: 29021740] (ICC/IF, Rat) ICC/IF Rat
El Yamani FZ, Yon L, Guerin M, El Ouezzani S et al. Immunocytochemical distribution of EM66 within the hypothalamic parvocellular paraventricular nucleus: Colocalization with CRH and TRH but no plasticity related to acute stress and thyroidectomy in the rat. Regul Pept 2013 Jan 16 [PMID: 23333484] (IHC, ICC/IF, Rat) IHC, ICC/IF Rat

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol CRH
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