Corticotropin Releasing Factor Antibody - Azide and BSA Free

Immunohistochemistry: Corticotropin Releasing Factor Antibody [NB110-81721] - Immunohistochemical detection of Corticotropin Releasing Factor in Rat Hypothalamic Brain Tissue using a 1ug/ml dilution of Affinity Purified Sheep Anti-CRF . Left is tested using antibody. Right is testing using negative control antibody.

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Corticotropin Releasing Factor Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Sheep Corticotropin Releasing Factor Antibody - Azide and BSA Free (NB110-81721) is a polyclonal antibody validated for use in IHC, ELISA and ICC/IF. Anti-Corticotropin Releasing Factor Antibody: Cited in 9 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: An 18 amino acid synthetic CRF peptide (H-CEQLAQQAHSNRKLMEII-OH), conjugated to Keyhole Limpet Hemocyanin (KLH).
• Specificity: Specific for Corticotropin Releasing Factor. Confirmed by Immunohistochemical analysis of Paraffin Embedded Rat Brain (Hypothalamus).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CRH
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:10
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Control:

  Corticotropin Releasing Factor Overexpression Lysate

Reactivity Notes

This antibody recognises CRF in Human, Rat and Mouse. There is no Cross-reactivity with Guinea Pig.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.05X PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with 0.02 ml sterilized water.

Alternate Names for Corticotropin Releasing Factor Antibody - Azide and BSA Free

• corticoliberin
• corticotropin releasing hormone
• Corticotropin-releasing hormone
• CRFCorticotropin-releasing factor

Background

Corticotropin-releasing hormone (CRH) is a 41-amino acid peptide derived from a 191-amino acid preprohormone. CRH issecreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in CRH hasbeen observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin dificiency hasmultiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to productionin the hypothalamus, CRH is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed inthe placenta. In the placenta CRH is a marker that determines the length of gestation and the timing of parturitionand delivery. A rapid increase in circulating levels of CRH occurs at the onset of parturition, suggesting that, inaddition to its metabolic functions, CRH may act as a trigger for parturition. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Corticotropin Releasing Factor Antibody (NB110-81721)(9)

Publications using NB110-81721

• Chen Z, Zhou T, Li Y et al. Paraventricular Mast Cell-Derived Histamine Activates CRH Neurons to Mediate Adult Visceral Hypersensitivity Induced by Neonatal Maternal Separation Brain sciences 2023-11-16 [PMID: 38002554]
• Wang Y, Zhang Y, Shi Z et al. Exposure of male mice to perfluorooctanoic acid induces anxiety-like behaviors by increasing corticotropin-releasing factor in the basolateral amygdala complex Chemosphere 2022-01-01 [PMID: 34826932] (IF/IHC, Mouse)
• Nass SR, Lark ARS, Hahn YK et al. HIV-1 Tat and morphine decrease murine inter-male social interactions and associated oxytocin levels in the prefrontal cortex, amygdala, and hypothalamic paraventricular nucleus Hormones and behavior 2021-06-22 [PMID: 34171549]
• Song Y, Meng Q, Wu K et al. Disinhibition of PVN-projecting GABAergic neurons in AV region in BNST participates in visceral hypersensitivity in rats Psychoneuroendocrinology 2020-01-01 [PMID: 32417623] (IF/IHC, Rat)
• Aguiniga LM, Searl TJ, Rahman-Enyart A et al. Acyloxyacyl hydrolase modulates voiding activity Am. J. Physiol. Renal Physiol. 2020-01-31 [PMID: 32003596]
• Aguiniga, LM;Yang, W;Yaggie, RE;Schaeffer, AJ;Klumpp, DJ;, ; ACYLOXYACYL HYDROLASE MODULATES DEPRESSIVE-LIKE BEHAVIORS THROUGH ARYL HYDROCARBON RECEPTOR Am. J. Physiol. Regul. Integr. Comp. Physiol. 2019-04-24 [PMID: 31017816] (IHC-P, Mouse)
• Lee B, Shim I, Lee H et al. Tetramethylpyrazine reverses anxiety-like behaviors in a rat model of post-traumatic stress disorder Korean J Physiol Pharmacol 2018-08-29 [PMID: 30181699] (ELISA, Rat)
• Zhu J, Chen Z, Meng Z et al. Electroacupuncture Alleviates Surgical Trauma-Induced Hypothalamus Pituitary Adrenal Axis Hyperactivity Via microRNA-142. Front Mol Neurosci. 2017-10-12 [PMID: 29021740] (ICC/IF, Rat)
• El Yamani FZ, Yon L, Guerin M, El Ouezzani S et al. Immunocytochemical distribution of EM66 within the hypothalamic parvocellular paraventricular nucleus: Colocalization with CRH and TRH but no plasticity related to acute stress and thyroidectomy in the rat. Regul Pept 2013-01-16 [PMID: 23333484] (ICC/IF, IF/IHC, Rat)

Bioinformatics

• Gene Symbol: CRH
• Entrez:
  • 1392(Human)
  • 12918(Mouse)
  • 81648(Rat)