Cortactin Overexpression Lysate (Native)

Images

 
Western Blot: Cortactin Overexpression Lysate (Adult Normal) [NBP2-04417] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cortactin.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Cortactin Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_612632. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens Cortactin, mRNA.
Gene
CTTN

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
57.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Cortactin

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Cortactin Overexpression Lysate (Native)

  • 1110020L01Rik
  • Amplaxin
  • Cortactin
  • CTTN
  • ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 srcsubstrate)
  • EMS1
  • EMS1amplaxin
  • FLJ34459
  • Oncogene EMS1
  • src substrate cortactin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF2685
Species: Hu
Applications: WB, Simple Western, IHC
H00010097-M01
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP2-32840
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC-P, IP
AF1444
Species: Mu
Applications: WB, IHC
NBP1-30955
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
H00010096-M02
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-45923
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
AF3854
Species: Hu, Mu, Rt
Applications: WB, IHC, ICC
NB200-603
Species: Hu, Mu, Xp
Applications: WB, IHC, IHC-Fr, IHC-P, IP, IF
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB100-2307
Species: Hu
Applications: WB
AF4259
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC
AF5789
Species: Hu, Mu, Rt
Applications: WB
NB100-91266
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, ICC
AF7035
Species: Hu
Applications: IHC
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-04417
Species: Hu
Applications: WB

Publications for Cortactin Lysate (NBP2-04417) (0)

There are no publications for Cortactin Lysate (NBP2-04417).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Cortactin Lysate (NBP2-04417) (0)

There are no reviews for Cortactin Lysate (NBP2-04417). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Cortactin Lysate (NBP2-04417) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Cortactin Products

Bioinformatics Tool for Cortactin Lysate (NBP2-04417)

Discover related pathways, diseases and genes to Cortactin Lysate (NBP2-04417). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Cortactin Lysate (NBP2-04417)

Discover more about diseases related to Cortactin Lysate (NBP2-04417).
 

Pathways for Cortactin Lysate (NBP2-04417)

View related products by pathway.

PTMs for Cortactin Lysate (NBP2-04417)

Learn more about PTMs related to Cortactin Lysate (NBP2-04417).
 

Research Areas for Cortactin Lysate (NBP2-04417)

Find related products by research area.

Blogs on Cortactin

There are no specific blogs for Cortactin, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Cortactin Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol CTTN
Entrez