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Cortactin Antibody (SC61-08)

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Western Blot: Cortactin Antibody (SC61-08) [NBP2-67632] - Analysis of Cortactin on different lysates using anti-Cortactin antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: A431
Western Blot: Cortactin Antibody (SC61-08) [NBP2-67632] - Analysis of Cortactin on different lysates using anti-Cortactin antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: A431
Immunocytochemistry/ Immunofluorescence: Cortactin Antibody (SC61-08) [NBP2-67632] - Staining Cortactin in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry-Paraffin: Cortactin Antibody (SC61-08) [NBP2-67632] - Analysis of paraffin-embedded mouse colon tissue using anti-Cortactin antibody. Counter stained with hematoxylin.
Flow Cytometry: Cortactin Antibody (SC61-08) [NBP2-67632] - Analysis of Hela cells with Cortactin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; ...read more
Immunocytochemistry/ Immunofluorescence: Cortactin Antibody (SC61-08) [NBP2-67632] - Staining Cortactin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunocytochemistry/ Immunofluorescence: Cortactin Antibody (SC61-08) [NBP2-67632] - Staining Cortactin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, ...read more
Immunohistochemistry-Paraffin: Cortactin Antibody (SC61-08) [NBP2-67632] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cortactin antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Cortactin Antibody (SC61-08) [NBP2-67632] - Analysis of paraffin-embedded human tonsil tissue using anti-Cortactin antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
SC61-08
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Cortactin Antibody (SC61-08) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Human Cortactin aa 1-200
Localization
Cytoplasm, Cell projection, Membrane.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CTTN
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:10 - 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Alternate Names for Cortactin Antibody (SC61-08)

  • 1110020L01Rik
  • Amplaxin
  • Cortactin
  • CTTN
  • ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 srcsubstrate)
  • EMS1
  • EMS1amplaxin
  • FLJ34459
  • Oncogene EMS1
  • src substrate cortactin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF2685
Western blot shows lysates of MDA‑MB‑468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 50 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Src (Y419) at approximately 56 - 61 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Src phosphorylated at Y419 was detected in immersion fixed paraffin-embedded sections of human liver array using Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western lane view shows lysates of MBA‑MB‑468 human breast cancer cell line untreated (-) or treated (+) with 50 µM Pervanadate (PV) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Src (Y419) at approximately 64 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
(1 Review)
(5 Publications)
Species: Hu
Applications: WB, Simple Western, IHC
ACTR2 Antibody
(1B10-C4)
H00010097-M01
Western Blot: ACTR2 Antibody (1B10-C4) [H00010097-M01] - ACTR2 monoclonal antibody (M01), clone 1B10-C4 Analysis of ACTR2 expression in Hela S3 NE.
Immunohistochemistry-Paraffin: ACTR2 Antibody (1B10-C4) [H00010097-M01] - Analysis of monoclonal antibody to ACTR2 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml.
Western Blot: ACTR2 Antibody (1B10-C4) [H00010097-M01] - Western Blot analysis of ACTR2 expression in A-431 ( Cat # L015V1 ).
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
Cyclin D1 Antibody
(SPM587)
NBP2-32840
Western Blot: Cyclin D1 Antibody (SPM587) [NBP2-32840] - Western Blot of cyclin D1 in (1) C2C12 (2) HepG2 & (3) NIH3T3 Lysate with Cyclin D1 Ab (SPM587)
Immunohistochemistry-Paraffin: Cyclin D1 Antibody (SPM587) [NBP2-32840] - Formalin-paraffin human Mantle Cell Lymphoma stained with Cyclin D1 Ab (Clone SPM587).
Western Blot: Cyclin D1 Antibody (SPM587) [NBP2-32840] - analysis of Cyclin D1 in human breast cancer cell line MDA-MB-231 cell lysate using anti-Cyclin D1. Image from verified customer review.
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Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Angiopoietin-like
Protein 2/ANGPTL2 Ant ...
AF1444
Western blot shows lysates of mouse uterus tissue and MEF mouse embryonic feeder cells. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse Angiopoietin‑like Protein 2/ANGPTL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1444) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Angiopoietin‑like Protein 2/ANGPTL2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Species: Mu
Applications: WB, IHC
AICDA Antibody
NBP1-30955
Western Blot: AICDA Antibody [NBP1-30955] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE, antibody diluted at 1:500.
Immunocytochemistry/Immunofluorescence: AICDA Antibody [NBP1-30955] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: AICDA Antibody [NBP1-30955] - Paraffin-embedded mouse lymph node. AICDA antibody [N1N2], N-term dilution: 1:500.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
ACTR3 Antibody
(JB33-44)
NBP2-75395
Western Blot: ACTR3 Antibody (JB33-44) [NBP2-75395] - Analysis of Arp3 on different lysates using anti-Arp3 antibody at 1/500 dilution.Positive control:Lane 1: A431 Lane 2: Mouse placentaLane 3: Mouse thymus
Immunocytochemistry/Immunofluorescence: ACTR3 Antibody (JB33-44) [NBP2-75395] - Staining Arp3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: ACTR3 Antibody (JB33-44) [NBP2-75395] - Analysis of paraffin-embedded rat kidney tissue using anti-Arp3 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
FAK Antibody
(SR46-04)
NBP2-67327
Western Blot: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of FAK on different lysates using anti-FAK antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Mouse spleen
Immunocytochemistry/Immunofluorescence: FAK Antibody (SR46-04) [NBP2-67327] - Staining FAK in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: FAK Antibody (SR46-04) [NBP2-67327] - Analysis of paraffin-embedded huamn liver cancer tissue using anti-FAK antibody. Counter stained with hematoxylin.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
EGFR Antibody
NBP1-84814
Western Blot: EGFR Antibody [NBP1-84814] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/Immunofluorescence: EGFR Antibody [NBP1-84814] - Staining of human cell line A-431 shows positivity in plasma membrane. Antibody staining is shown in green.
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N-WASP Antibody
NBP1-82512
Western Blot: N-WASP Antibody [NBP1-82512] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/Immunofluorescence: N-WASP Antibody [NBP1-82512] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining in human cerebral cortex and skeletal muscle tissues. Corresponding WASL RNA-seq data are presented for the same tissues.
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Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
FGF-3 Antibody
(MSD1)
NB200-603
FGF-3 Antibody (MSD1)
(1 Review)
(1 Publication)
Species: Hu, Mu, Xp
Applications: WB, IHC, IHC-Fr, IHC-P, IP, IF
AKT1 [p Ser473]
Antibody (104A282)
NB100-56749
Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - Total protein from mouse 3T3 cells treated with and without PDGF (50 ng/mL) for the indicated times was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-AKT1 (NBP2-01725) and 2 ug/mL pS473 AKT1 in 1% BSA in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Note the detection of phosphorylated AKT1 in response to PDGF treatment compared to total AKT1 protein.
Western Blot: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - WB of phospho AKT using phospho AKT antibody at 2 ug/mL against untreated (lane 1) and PDGF treated (lane 2) NIH-3T3 lysate. HRP conjugated secondary antibody and ECL substrate solution were used for this test. Image using the Azide and BSA Free form of this antibody.
Immunohistochemistry-Paraffin: AKT1 [p Ser473] Antibody (104A282) [NB100-56749] - IHC analysis of an FFPE human breast carcinoma tissue section using 1:250 dilution of phospho Ser473 AKT1 antibody (clone 104A282) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of phosphor-AKT (Ser-473) in the cancer cells as well as the stromal cells. Staining was performed by Histowiz.
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WASP Antibody
(7B10E4)
NBP2-52587
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Immunohistochemistry-Paraffin: WASP Antibody (7B10E4) [NBP2-52587] - Analysis of paraffin-embedded colon cancer tissues using WAS mouse mAb with DAB staining.
Flow Cytometry: WASP Antibody (7B10E4) [NBP2-52587] - Analysis of Hela cells using WAS mouse mAb (green) and negative control (red).
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Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready
EGF [Unconjugated]
236-EG
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1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE  and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.
(15 Reviews)
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Paxillin [p
Tyr118] Antibody
NBP2-24459
Western Blot: Paxillin [p Tyr118] Antibody [NBP2-24459] - Analysis of phospho Paxillin (Y118) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 118 and 2) an unphosphorylated tyrosine at position 118, using paxillin phospho antibody at 0.025 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
(1 Publication)
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FGF-4
7460-F4/CF
FGF-4
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H00003059-M02
Western Blot: HCLS1 Antibody (1A8) [H00003059-M02] - HCLS1 monoclonal antibody (M02), clone 1A8 Analysis of HCLS1 expression in K-562.
Immunohistochemistry-Paraffin: HCLS1 Antibody (1A8) [H00003059-M02] - Analysis of monoclonal antibody to HCLS1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
Western Blot: HCLS1 Antibody (1A8) [H00003059-M02] - Analysis of HCLS1 over-expressed 293 cell line, cotransfected with HCLS1 Validated Chimera RNAi ( Cat # H00003059-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HCLS1 monoclonal antibody (M02), clone 1A8 (Cat # H00003059-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
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Rac1 Antibody
(JM11-29)
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Western Blot: Rac1 Antibody (JM11-29) [NBP2-67091] - Analysis of RAC1/2/3 on MCF-7 cells lysates using anti-RAC1/2/3 antibody at 1/500 dilution.
Immunocytochemistry/Immunofluorescence: Rac1 Antibody (JM11-29) [NBP2-67091] - Staining RAC1/2/3 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Rac1 Antibody (JM11-29) [NBP2-67091] - Analysis of paraffin-embedded human tonsil tissue using anti-RAC1/2/3 antibody. Counter stained with hematoxylin.
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SHANK2 Antibody
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Western Blot: SHANK2 Antibody (S23b-6) [NBP2-12914] - analysis of Rat brain membrane lysate showing detection of SHANK2 protein using Mouse Anti-SHANK2 Monoclonal Antibody, Clone S23b-6. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK2 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP, MiAr
ERK1 Antibody (1E5)
NBP2-22203
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.
Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
ERK2 Antibody
(SZ25-01)
NBP2-67360
Western Blot: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of ERK2 on different lysates using anti-ERK2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12
Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: ERK2 Antibody (SZ25-01) [NBP2-67360] - Analysis of paraffin-embedded mouse stomach tissue using anti-ERK2 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP

Publications for Cortactin Antibody (NBP2-67632) (0)

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