Cortactin Antibody (771716) [mFluor Violet 610 SE]

• Reactivity: Hu
• Applications: WB, IHC, KO
• Clone: 771716
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: mFluor Violet 610 SE

Cortactin Antibody (771716) [mFluor Violet 610 SE] Summary

• Immunogen: E. coli-derived recombinant human Cortactin
  Met1-Gly85
  Accession # Q14247
• Specificity: Detects human Cortactin in ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CTTN
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Knockout Validated
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Cortactin Antibody (771716) [mFluor Violet 610 SE]

• 1110020L01Rik
• Amplaxin
• Cortactin
• CTTN
• ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 srcsubstrate)
• EMS1
• EMS1amplaxin
• FLJ34459
• Oncogene EMS1
• src substrate cortactin

Background

Immunocytochemistry reveals that in epithelial cells, the human cortactin protein is localized mainly in the cytoplasm and, to a very low extent, in protruding leading lamellae of the cell. However, in carcinoma cells that constitutively overexpress cortactin accumulates in the podosome-like adherens junctions associated with the cell-substratum contact sites. Overexpression and concomitant accumulation of the Cortactin protein in the cell-substratum contact sites might, therefore, contribute to the invasive potential of these tumor cells (1). In cells derived from breast carcinomas and squamous carcinomas of the head and neck, DNA amplification of a particular tyrosine kinase substrate region results in overexpression of cortactin. Overexpression is accompanied by a partial redistribution of cortactin from the cytoplasm into cell-matrix contact sites (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CTTN