COP9 signalosome complex subunit 2 Antibody

Western Blot: COP9 signalosome complex subunit 2 Antibody [NB100-1238] - 3T3 lysates (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemistry: COP9 signalosome complex subunit 2 Antibody [NB100-1238] - In paraffin embedded Human Thyroid Gland shows nclear and cytoplasm staining in activated epithelial cells Recommended concentration 3-5/ugml.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

COP9 signalosome complex subunit 2 Antibody Summary

• Description: Novus Biologicals Goat COP9 signalosome complex subunit 2 Antibody (NB100-1238) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-COP9 signalosome complex subunit 2 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-LNSLNQAVVSKLA corresponding to C-Terminus according to NP_001137359.1.
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: COPS2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 48 kDa band observed in human brain, mouse brain lysates and in lysates of mouse putuitary cell line NIH3T3 (calculated MW of 51.6 kDa band according to NP_004227.1). IHC-P: Human thyroid gland shows nclear and cytoplasm staining in activated epithelial cells
• Control:

  COP9 signalosome complex subunit 2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for COP9 signalosome complex subunit 2 Antibody

• Alien homolog
• ALIEN
• COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis)
• COP9 signalosome complex subunit 2
• CSN2JAB1-containing signalosome subunit 2
• SGN2TR-interacting protein 15
• Signalosome subunit 2
• thyroid receptor interacting protein 15
• Thyroid receptor-interacting protein 15
• TRIP15TRIP-15

Background

TRIP15 is an essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP. TRIP15 is also involved in early stage of neuronal differentiation via its interaction with NIF3L1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for COP9 signalosome complex subunit 2 Antibody (NB100-1238)(2)

Publications using NB100-1238

• Besse A, Campos AD, Webster WK, Darnay BG. TRAF-interacting protein (TRIP) is a RING-dependent ubiquitin ligase. Biochem Biophys Res Commun. 2007-08-03 [PMID: 17544371]
• Escher N, Kob R, Tenbaum SP et al. Various members of the E2F transcription factor family interact in vivo with thecorepressor alien. J Proteome Res 2007-03-01 [PMID: 17330949]

Bioinformatics

• Gene Symbol: COPS2
• Entrez:
  • 9318(Human)
  • 12848(Mouse)
  • 261736(Rat)
• Uniprot:
  • NP_004227.(Human)