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COP9 signalosome complex subunit 2 Overexpression Lysate

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Western Blot: COP9 signalosome complex subunit 2 Overexpression Lysate (Adult Normal) [NBL1-09380] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for COP9 signalosome complex subunit 2.
Western Blot: COP9 signalosome complex subunit 2 Overexpression Lysate (Adult Normal) [NBL1-09380] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for COP9 ...read more

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Summary
Reactivity HuSpecies Glossary
Applications WB

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COP9 signalosome complex subunit 2 Overexpression Lysate Summary

Description
Transient overexpression lysate of COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis) (COPS2), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC203159 and based on accession number NM_004236. The protein contains a C-MYC/DDK Tag.
Gene
COPS2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
51.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for COP9 signalosome complex subunit 2 Overexpression Lysate

  • Alien homolog
  • ALIEN
  • COP9 constitutive photomorphogenic homolog subunit 2 (Arabidopsis)
  • COP9 signalosome complex subunit 2
  • CSN2JAB1-containing signalosome subunit 2
  • SGN2TR-interacting protein 15
  • Signalosome subunit 2
  • thyroid receptor interacting protein 15
  • Thyroid receptor-interacting protein 15
  • TRIP15TRIP-15

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Immunohistochemistry-Paraffin: CSN3 Antibody [NBP1-85436] - Staining of human colon, lactating breast, liver and testis using Anti-CSN3 antibody NBP1-85436 (A) shows similar protein distribution across tissues to independent antibody NBP2-58781 (B).
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Western Blot: COP9 Antibody (JE47-48) [NBP2-80456] - Analysis of COPS3/CSN3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human skeletal muscle tissue lysate Lane 2: Rat bone marrow lysate.
Immunocytochemistry/Immunofluorescence: COP9 Antibody (JE47-48) [NBP2-80456] - ICC staining of COPS3/CSN3 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1:500) for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:100 dilution. The nuclear counter stain is DAPI (blue).
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COP9 signalosome
complex subunit 2 Ove ...
NBL1-09380
COP9 signalosome complex subunit 2 Overexpression Lysate
Species: Hu
Applications: WB

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Bioinformatics

Gene Symbol COPS2