Contactin-2/TAG1 Antibody (372913) [Unconjugated]

Western blot shows lysates of human cerebellum tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Contactin-2/TAG1 Monoclonal Antibody (Catalog # MAB17141) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Contactin-2/TAG1 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 372913
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Contactin-2/TAG1 Antibody (372913) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Contactin‑2/TAG1
  Leu29-Asn1012
  Accession # Q02246
• Specificity: Detects human Contactin‑2/TAG1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Contactin‑4, rhContactin‑5, recombinant mouse (rm) Contactin‑2, and rmContactin‑3 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CNTN2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Contactin-2/TAG1 Antibody (372913) [Unconjugated]

• Axonal glycoprotein TAG-1
• axonin-1 cell adhesion molecule
• Axonin-1
• AXT
• CNTN2
• contactin 2 (axonal)
• contactin 2 (transiently expressed)
• Contactin2
• Contactin-2
• DKFZp781D102
• FLJ37193
• MGC157722
• TAG1
• TAG-1
• TAX
• TAX1
• TAX-1
• TAX1FLJ42746
• Transient axonal glycoprotein 1
• transiently-expressed axonal glycoprotein

Background

Contactin-2 (CNTN2), also called TAG-1 (transient axonal glycoprotein), TAX1 (transiently-expressed axonal glycoprotein), or axonin-1, is a 135 kDa glycosyl‑phosphatidylinositol (GPI)- anchored cell adhesion molecule that belongs to the contactin subfamily within the immunoglobulin (Ig) protein superfamily (1‑3). Human Contactin-2 cDNA encodes a 28 amino acid (aa) signal peptide, a 984 aa mature secreted protein with six Ig-like domains followed by four fibronectin type III‑like repeats, and a 28 aa C-terminal GPI anchor pro-sequence. GPI-specific phospholipase activity can release soluble, active Contactin-2 from the membrane (2). Mature human Contactin-2 shares approximately 93%, 93% and 75% aa sequence identity with human, rat and chicken Contactin-2, respectively. During development, Contactin-2 is expressed by a subset of neuronal populations in the central nervous system (CNS) and peripheral nervous system (PNS), particularly during initial phases of axon outgrowth (3‑5). Both the 135 kDa form and a 90 kDa form are also upregulated in response to CNS injury in the adult (6). Data support a role for Contactin-2 in axon pathfinding, neurite outgrowth and adhesion, especially in the CNS (36). In mature myelinated fibers, Contactin-2 is expressed by oligodendrocytes and Schwann cells, which are myelinating glial cells of the CNS and PNS, respectively (7, 8). It is enriched in the juxtaparanodal regions, where it recruits caspr2 (Contactin-associated protein 2), a transmembrane neurexin involved in cell adhesion and intercellular communication (7‑10). The axonal Contactin-2 interacts in cis with caspr2, and in trans with another Contactin-2 on the glial membrane (8). This ternary complex is required for the accumulation and organization of K⁺ channels in the juxtaparanodes (9).

1. Wolfer, D. & R. J. Giger (1994) Swissprot accession # Q61330.
2. Hasler, T.H. et al. (1993) Eur. J. Biochem. 211:329.
3. Karagogeos, D. (2003) Front. Biosci.8:s1304.
4. Liu, Y. & M.C. Halloran (2005) J. Neurosci. 25:10556.
5. Denaxa, M. et al. (2005) Dev. Biol. 288:87.
6. Soares, S. et al. (2005) Eur. J. Neurosci. 21:1169.
7. Traka, M. et al. (2002) J. Neurosci. 22:3016.
8. Poliak, S. and E. Peles (2003) Nature Reviews Neurosci. 4:968.
9. Traka, M. et al. (2003) J. Cell Biol. 162:1161.
10. Poliak, S. et al. (2003) J. Cell Biol. 162:1149.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Contactin-2/TAG1 Antibody (MAB17141)(6)

Publications using MAB17141

• Gusar, V;Kan, N;Leonova, A;Chagovets, V;Tyutyunnik, V;Khachatryan, Z;Yarotskaya, E;Sukhikh, G; Non-Invasive Assessment of Neurogenesis Dysfunction in Fetuses with Early-Onset Growth Restriction Using Fetal Neuronal Exosomes Isolating from Maternal Blood: A Pilot Study International journal of molecular sciences 2025-02-11 [PMID: 40003962] (Immunoprecipitation, Human)
• Darbinian, N;Merabova, N;Tatevosian, G;Morrison, M;Darbinyan, A;Zhao, H;Goetzl, L;Selzer, ME; Biomarkers of Affective Dysregulation Associated with In Utero Exposure to EtOH Cells 2023-12-19 [PMID: 38201206] (Immunoprecipitation, Human)
• Darbinian, N;Gallia, GL;Darbinyan, A;Vadachkoria, E;Merabova, N;Moore, A;Goetzl, L;Amini, S;Selzer, ME; Effects of In Utero EtOH Exposure on 18S Ribosomal RNA Processing: Contribution to Fetal Alcohol Spectrum Disorder International journal of molecular sciences 2023-09-05 [PMID: 37762017] (Immunoprecipitation, Human)
• Darbinian, N;Darbinyan, A;Merabova, N;Kassem, M;Tatevosian, G;Amini, S;Goetzl, L;Selzer, ME; In utero ethanol exposure induces mitochondrial DNA damage and inhibits mtDNA repair in developing brain Frontiers in neuroscience 2023-08-09 [PMID: 37621718] (Immunoprecipitation, Human)
• N Darbinian, A Darbinyan, J Sinard, G Tatevosian, N Merabova, F D'Amico, T Khader, A Bajwa, D Martirosya, AK Gawlinski, R Pursnani, H Zhao, S Amini, M Morrison, L Goetzl, ME Selzer Molecular Markers in Maternal Blood Exosomes Allow Early Detection of Fetal Alcohol Spectrum Disorders International Journal of Molecular Sciences, 2022-12-21;24(1):. 2022-12-21 [PMID: 36613580] (Flow Cytometry, Human)
• van Nierop G, Janssen M, Mitterreiter J, van de Vijver D, de Swart R, Haagmans B, Verjans G, Hintzen R Intrathecal CD4(+) and CD8(+) T-cell responses to endogenously synthesized candidate disease-associated human autoantigens in multiple sclerosis patients. Eur J Immunol, 2015-11-09;46(2):347-53. 2015-11-09 [PMID: 26507805] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CNTN2
• Uniprot:
  • Q02246()