Contactin-1 Antibody [Unconjugated]

Western blot shows lysates of human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and rat brain tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat Contactin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7549) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Contactin-1 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Contactin-1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Human/Mouse/Rat Contactin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7549) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal root ganglia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Contactin-1 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Contactin-1
  Asp21-Thr999
  Accession # AAH66864
• Specificity: Detects human, mouse, and rat Contactin-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Contactin-2 and rmContactin-4 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CNTN1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Contactin-1 Antibody [Unconjugated]

• CNTN1
• contactin 1
• Contactin1
• Contactin-1
• F11
• F3
• F3cam
• Glycoprotein gp135
• GP135
• Neural cell surface protein F3

Background

CNTN1 (Contactin-1; also neuronal cell surface protein F3/F11) is a 135-145 kDa GPI-linked glycoprotein member of the Contactin family, Ig superfamily of molecules. It is expressed on the surface of oligodendrocytes and neurons, principally on axons, and has multiple binding partners. In particular, it binds to astrocyte tenascin-C, Schwann cell NF/neurofascin-155, and oligodendrocyte produced tenascin-R and Notch in-trans, plus paranodin in-cis. Tenascin-R interactions inhibit neurite outgrowth, paranodin/NF155 interactions may contribute to Ranvier node development, and Notch ligation results in oligodendrocyte maturation. CNTN1 also binds to chondroitin sulfate-E, an interaction that initiates neurite outgrowth. Mouse CNTN1 is synthesized as a 1020 amino acid (aa) preproprecursor (aa 1-1020). It contains a 20 aa signal sequence, followed by a 981 aa mature region (aa 21-1001), and a 19 aa propeptide. The mature region possesses six consecutive C2-type Ig‑like domains (aa 41-603) that regulate neurite outgrowth, followed by three FN-type III domains (aa 605-901) that regulate adhesiveness. It is estimated that 15% of the native MW constitutes carbohydrate, part of which is represented by the HNK-1 antigen (3-sulfo-GluA-Gal-GlcNac). Over aa 20-999, mouse CNTN1 shares 96% and 99% aa sequence identity with human and rat CNTN1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Contactin-1 Antibody (AF7549)(1)

Publication using AF7549

• N Apóstolo, SN Smukowski, J Vanderlind, G Condomitti, V Rybakin, J Ten Bos, L Trobiani, S Portegies, KM Vennekens, NV Gounko, D Comoletti, KD Wierda, JN Savas, J de Wit Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer Nat Commun, 2020-10-14;11(1):5171. 2020-10-14 [PMID: 33057002] (IHC, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CNTN1
• Uniprot:
  • AAH66864()